The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr.
The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr.
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DOI：
10.3390/molecules21010090
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发表时间：
2016-01-14
期刊：
影响因子：
--
通讯作者：
Świeboda R
中科院分区：
文献类型：
--
作者：
Wójciak-Kosior M;Sowa I;Blicharski T;Strzemski M;Dresler S;Szymczak G;Wnorowski A;Kocjan R;Świeboda R
植物体内次生代谢物的数量可因各种外界因素的影响而增加或减少。本研究探讨了锶离子对大豆中植物雌激素产生的影响。植株用Hoagland‘s溶液处理，用浓度为0.5~3.0 mM的Sr2+进行修饰，在水培条件下培养14天。收获后，大豆植株被分成根和枝，干燥，并粉碎。用甲醇提取植物性物质，并对其进行水解性研究。用高效液相色谱法测定植物雌激素的含量。与对照相比，在所有测试浓度的锶离子中观察到感兴趣的化合物的浓度显著增加。浓度为2 mM的Sr2+是最强的诱导剂，大豆苷元、香豆素、染料木素和芒柄花素的植物雌激素含量分别增加了2.70、1.92、3.77和2.88倍。此外，与非胁迫植物的提取物相比，经2 mMSr2+胁迫的大豆植物提取物处理后，在HepG2肝细胞模型中没有观察到细胞毒效应。我们的结果表明，在培养基中添加锶离子可用于提高植物雌激素含量的大豆植株的功能化。
The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland’s solution, modified with Sr2+ with concentrations ranging from 0.5 to 3.0 mM, and were grown for 14 days in hydroponic cultivation. After harvest, soybean plants were separated into roots and shoots, dried, and pulverized. The plant material was extracted with methanol and hydrolyzed. Phytoestrogens were quantified by HPLC. The significant increase in the concentration of the compounds of interest was observed for all tested concentrations of strontium ions when compared to control. Sr2+ at a concentration of 2 mM was the strongest elicitor, and the amount of phytoestrogens in plant increased ca. 2.70, 1.92, 3.77 and 2.88-fold, for daidzein, coumestrol, genistein and formononetin, respectively. Moreover, no cytotoxic effects were observed in HepG2 liver cell models after treatment with extracts from 2 mM Sr2+-stressed soybean plants when compared to extracts from non-stressed plants. Our results indicate that the addition of strontium ions to the culture media may be used to functionalize soybean plants with enhanced phytoestrogen content.
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影响因子：
4
作者：
Xiao, Jun-Xia;Huang, Guo-Qing;Qiu, Hong-Wei
通讯作者：
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影响因子：
2.2
作者：
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影响因子：
2.3
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
5.1
作者：
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通讯作者：
Momparler, Richard L.