Fine-scale population epigenetic structure in relation to gastrointestinal parasite load in red grouse (Lagopus lagopus scotica).
Fine-scale population epigenetic structure in relation to gastrointestinal parasite load in red grouse (Lagopus lagopus scotica).
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DOI：
10.1111/mec.12833
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发表时间：
2014-09
影响因子：
4.9
通讯作者：
Piertney SB
中科院分区：
文献类型：
--
作者：
Wenzel MA;Piertney SB
胞嘧啶甲基化状态的表观遗传修饰可以由环境胁迫引起，并且可能是影响表型可塑性和适应性的关键过程。寄生虫是对宿主具有深刻生理和生态影响的强有力的应激源，但对寄生虫如何影响宿主甲基化状态知之甚少。在这里，我们估计表观遗传多样性和分化的21个种群的红鸡（Lagopus lagopus scotica）在苏格兰东北部和胃肠道寄生虫负荷（盲肠线虫细毛圆线虫）与肝脏全基因组和位点特异性甲基化状态的关联测试。甲基化敏感的AFLP（MSAP），129条带，代表73个甲基化敏感和56个非甲基化的epiloci，在234个人进行了评分。这些群体在全基因组甲基化水平上存在显著差异，并且在表观遗传学（FSC = 0.0227; P < 0.001）和遗传学（FSC = 0.0058; P < 0.001）上也存在显著分化。寄生虫负荷与全基因组甲基化水平或表观遗传分化无关。相反，我们发现了8个不成比例的分化癫痫特异性甲基化状态（FST离群值），使用贝叶斯软件和35个甲基化状态与寄生虫负荷的显着正相关和负相关，从定制的广义估计方程（GEE），简单逻辑回归（SAM）和贝叶斯环境分析（Bayenv 2）。经过桑格测序、基因组作图和遗传学（go）注释，这些epiloci中的一些与参与细胞周期、信号传导、代谢、免疫系统调节的基因相关，特别是rRNA甲基化、组蛋白乙酰化和小RNA。这些发现表明了野鸟种群中寄生虫负载的表观遗传特征，并表明寄生虫相关胞嘧啶甲基化的有趣生理效应。
Epigenetic modification of cytosine methylation states can be elicited by environmental stresses and may be a key process affecting phenotypic plasticity and adaptation. Parasites are potent stressors with profound physiological and ecological effects on their host, but there is little understanding in how parasites may influence host methylation states. Here, we estimate epigenetic diversity and differentiation among 21 populations of red grouse (Lagopus lagopus scotica) in north-east Scotland and test for association of gastrointestinal parasite load (caecal nematode Trichostrongylus tenuis) with hepatic genome-wide and locus-specific methylation states. Following methylation-sensitive AFLP (MSAP), 129 bands, representing 73 methylation-susceptible and 56 nonmethylated epiloci, were scored across 234 individuals. The populations differed significantly in genome-wide methylation levels and were also significantly epigenetically (FSC = 0.0227; P < 0.001) and genetically (FSC = 0.0058; P < 0.001) differentiated. Parasite load was not associated with either genome-wide methylation levels or epigenetic differentiation. Instead, we found eight disproportionately differentiated epilocus-specific methylation states (FST outliers) using bayescan software and significant positive and negative association of 35 methylation states with parasite load from bespoke generalized estimating equations (GEE), simple logistic regression (sam) and Bayesian environmental analysis (bayenv2). Following Sanger sequencing, genome mapping and geneontology (go) annotation, some of these epiloci were linked to genes involved in regulation of cell cycle, signalling, metabolism, immune system and notably rRNA methylation, histone acetylation and small RNAs. These findings demonstrate an epigenetic signature of parasite load in populations of a wild bird and suggest intriguing physiological effects of parasite-associated cytosine methylation.
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DOI：
10.1073/pnas.0610410104
发表时间：
2007-04-03
影响因子：
11.1
作者：
Crews, David;Gore, Andrea C.;Skinner, Michael K.
通讯作者：
Skinner, Michael K.
影响因子：
3.7
作者：
Boks MP;Derks EM;Weisenberger DJ;Strengman E;Janson E;Sommer IE;Kahn RS;Ophoff RA
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Ophoff RA
影响因子：
3.7
作者：
Bortolotti, Gary R.;Mougeot, Francois;Piertney, Stuart B.
通讯作者：
Piertney, Stuart B.
影响因子：
4.4
作者：
Caballero A;García-Pereira MJ;Quesada H
通讯作者：
Quesada H
DOI：
10.1098/rspb.2010.1211
发表时间：
2011-01-22
影响因子：
4.7
作者：
Berenos, Camillo;Wegner, K. Mathias;Schmid-Hempel, Paul
通讯作者：
Schmid-Hempel, Paul