Genome-based analysis of infrequent Salmonella serotypes through the Thai pork production chain.
Genome-based analysis of infrequent Salmonella serotypes through the Thai pork production chain.
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DOI：
10.3389/fmicb.2022.968695
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
沙门氏菌是一种流行的人畜共患食源性病原体。猪和猪肉被认为是人类沙门氏菌病的重要来源。在泰国北部的清迈府和南奔府，沙门氏菌的流行率十多年来一直居高不下。感染通常是肠沙门氏菌的主要血清型，包括 Rissen 和 1,4,[5],12:i:- 血清型。然而，其他血清型也导致疾病，但特征不太清楚。利用2011-2014年猪肉生产链中分离的43个肠沙门氏菌血清型的全基因组测序数据，基于核心基因组多位点序列分型（cgMLST）方法评估遗传多样性并确定沙门氏菌污染的可能来源。从农场和屠宰场回收的沙门氏菌血清型因猪环境污染而重新循环。相反，零售市场的沙门氏菌污染代表了多种来源的交叉污染，包括受污染的食品。猪肉生产链中的沙门氏菌污染具有宿主细胞粘附、宿主细胞侵袭和细胞内存活的能力，这足以产生沙门氏菌病的致病性。此外，所有这些分离株均为多重耐药沙门氏菌，其中含有至少 10 个抗菌素耐药基因。这一结果表明这些肠沙门氏菌血清型也构成重大的公共卫生风险。我们的研究结果支持需要对进入市场的食用动物产品进行适当的监测，以减少公众接触高致病性、多重耐药性沙门氏菌。获取信息将激励所有利益相关者加强整个猪肉生产链的卫生标准，以消除沙门氏菌污染并降低人类感染沙门氏菌病的风险。
Salmonella is a prevalent zoonotic foodborne pathogen. Swine and pork are implicated as important sources of salmonellosis in humans. In Chiang Mai and Lamphun Provinces in northern Thailand, there has been a high prevalence of Salmonella persistence for over a decade. Infection is usually with dominant S. enterica serotypes, including serotypes Rissen and 1,4,[5],12:i:-. However, other serotypes also contribute to disease but are less well characterized. The whole genome sequencing data of 43 S. enterica serotypes isolated from pork production chain through 2011–2014, were used to evaluate genetic diversity and ascertain the possible source of Salmonella contamination based on Core Genome Multilocus Sequence Typing (cgMLST) approach. The Salmonella serotypes recovered from farms and slaughterhouses were re-circulating by swine environmental contamination. Conversely, the Salmonella contamination in the retail market represents cross-contamination from multiple sources, including contaminated foodstuffs. Salmonella contamination in the pork production chain has the competency for host cell adhesion, host cell invasion, and intracellular survival, which is enough for the pathogenicity of salmonellosis. In addition, all of these isolates were multi-drug resistant Salmonella, which contained at least 10 antimicrobial resistance genes. This result indicated that these S. enterica serotypes also pose a significant public health risk. Our findings support the need for appropriate surveillance of food-animal products going to market to reduce public exposure to highly pathogenic, multi-drug resistant Salmonella. Acquiring information would motivate all stakeholders to reinforce sanitation standards throughout the pork production chain in order to eradicate Salmonella contamination and reduce the risk of salmonellosis in humans.
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