Broad and long-lasting immune protection against various Chikungunya genotypes demonstrated by participants in a cross-sectional study in a Cambodian rural community.
Broad and long-lasting immune protection against various Chikungunya genotypes demonstrated by participants in a cross-sectional study in a Cambodian rural community.
复制标题
DOI：
10.1038/s41426-017-0010-0
复制
发表时间：
2018-02-07
影响因子：
13.2
通讯作者：
Dussart P
中科院分区：
文献类型：
--
作者：
Auerswald H;Boussioux C;In S;Mao S;Ong S;Huy R;Leang R;Chan M;Duong V;Ly S;Tarantola A;Dussart P
基孔肯雅病毒（CHIKV）是一种在世界范围内传播的甲病毒。自20世纪50年代以来，它在亚洲的存在已被报道，构成了亚洲基因型。自2005年以来，来自东部、中部和南部非洲（ECSA）基因型的菌株在亚洲各地引起了几次疫情。2011年底，柬埔寨也发现了ECSA基因型病毒，并导致2012年农村社区爆发。2012年的一项调查发现，年龄小于40岁的人感染的风险更高，这表明柬埔寨老年人由于感染亚洲基因型而预先存在群体免疫力。2016年，我们收集了1975年之前出生和1980年之后出生的同等数量的人的血清，这些人也是2012年研究的一部分。我们分析了2016年的154份血清样本，以针对柬埔寨ECSA分离株和3株属于亚洲基因型的菌株进行中和。该实验显示，22.5%（18/80）的年轻研究参与者没有CHIKV抗体，而5.4%（4/74）的老年人群仍然是幼稚的。与其他两种亚洲基因型病毒相比，在ECSA爆发期间感染的研究参与者对ECSA和最古老的亚洲基因型病毒（泰国1958年）的中和滴度为两倍。中和数据也支持老年人群在1960年代暴露于亚洲基因型病毒。观察到的交叉反应性证实，尽管出现了新的ECSA基因型病毒，但所研究的CHIKV毒株属于单一血清型，并支持开发基孔肯雅疫苗的重要性。
Chikungunya virus (CHIKV) is an alphavirus circulating worldwide. Its presence in Asia has been reported since the 1950s, constituting the Asian genotype. Since 2005, strains from the Eastern, Central, and Southern African (ECSA) genotype have caused several outbreaks across Asia. Viruses from the ECSA genotype were also detected in Cambodia in late 2011 and led to an outbreak in a rural community in 2012. A former investigation from 2012 found a higher risk of infection in people younger than 40 years, suggesting a pre-existing herd immunity in the older Cambodian population due to infection with an Asian genotype. In 2016, we collected serum from equivalent numbers of individuals born before 1975 and born after 1980 that were also part of the 2012 study. We analyzed the 154 serum samples from 2016 for neutralization against the Cambodian ECSA isolate and three strains belonging to the Asian genotype. This experiment revealed that 22.5% (18/80) of the younger study participants had no CHIKV antibodies, whereas 5.4% (4/74) of the older population remained naive. Study participants infected during the ECSA outbreak had twofold neutralizing titers against the ECSA and the most ancient Asian genotype virus (Thailand 1958) compared to the other two Asian genotype viruses. The neutralization data also support the older population’s exposure to an Asian genotype virus during the 1960s. The observed cross-reactivity confirms that the investigated CHIKV strains belong to a single serotype despite the emergence of novel ECSA genotype viruses and supports the importance of the development of a Chikungunya vaccine.
登录
查看更多内容
DOI：
10.1016/0923-2516(96)80591-7
发表时间：
1995-01-01
期刊：
RESEARCH IN VIROLOGY
影响因子：
--
作者：
BLACKBURN, NK;BESSELAAR, TG;GIBSON, G
通讯作者：
GIBSON, G
影响因子：
7.8
作者：
KIELIAN, M;HELENIUS, A
通讯作者：
HELENIUS, A
影响因子：
6.7
作者：
Lee CY;Kam YW;Fric J;Malleret B;Koh EG;Prakash C;Huang W;Lee WW;Lin C;Lin RT;Renia L;Wang CI;Ng LF;Warter L
通讯作者：
Warter L
影响因子：
2.2
作者：
Imrie, Allison;Meeks, Janet;Effler, Paul
通讯作者：
Effler, Paul
影响因子：
11.8
作者：
AbuBakar, Sazaly;Sam, I-Ching;Roslan, Nuruliza
通讯作者：
Roslan, Nuruliza