Stepwise synthesis of giant unilamellar vesicles on a microfluidic assembly line.
Stepwise synthesis of giant unilamellar vesicles on a microfluidic assembly line.
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DOI：
10.1021/ja109137s
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发表时间：
2011-03-09
影响因子：
15
通讯作者：
Paegel BM
中科院分区：
文献类型：
--
作者：
Matosevic S;Paegel BM
Among the molecular milieu of the cell, the membrane bilayer stands out as a complex and elusive synthetic target. We report a microfluidic assembly line that produces uniform cellular compartments from droplet, lipid, and oil/water interface starting materials. Droplets form in a lipid-containing oil flow and travel to a junction where the confluence of oil and extracellular aqueous media establishes a flow-patterned interface that is both stable and reproducible. A triangular post mediates phase transfer bilayer assembly by deflecting droplets from oil, through the interface, and into the extracellular aqueous phase to yield a continuous stream of unilamellar phospholipid vesicles with uniform and tunable size. The size of the droplet precursor dictates vesicle size, encapsulation of small-molecule cargo is highly efficient, and the single bilayer promotes functional insertion of a bacterial transmembrane pore.
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影响因子：
56.9
作者：
Zhao, B;Moore, JS;Beebe, DJ
通讯作者：
Beebe, DJ
影响因子：
15
作者：
Jahn, A;Vreeland, WN;Locascio, LE
通讯作者：
Locascio, LE
影响因子：
5.6
作者：
BANGHAM, AD;STANDISH, MM;WATKINS, JC
通讯作者：
WATKINS, JC
影响因子：
64.8
作者：
Mansy, Sheref S.;Schrum, Jason P.;Szostak, Jack W.
通讯作者：
Szostak, Jack W.
DOI：
10.1073/pnas.81.19.6159
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRIAN, AA;MCCONNELL, HM
通讯作者：
MCCONNELL, HM