N-methyl-D-aspartate receptor subtype 3A promotes apoptosis in developing mouse brain exposed to hyperoxia.
N-methyl-D-aspartate receptor subtype 3A promotes apoptosis in developing mouse brain exposed to hyperoxia.
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DOI：
10.3969/j.issn.1673-5374.2012.04.006
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发表时间：
2012-02-05
影响因子：
6.1
通讯作者：
Wei L
中科院分区：
文献类型：
--
作者：
Li J;Yu S;Lu Z;Mohamad O;Wei L
在本研究中，出生后7天的C57/BL6野生型小鼠（高氧组）和出生后7天的N-甲基-D-天冬氨酸受体亚型3A敲除小鼠（NR3A KO组）在密闭容器中暴露于75%氧气和15%氮气5天。在常氧环境中饲养的野生型小鼠作为对照。 TdT介导的dUTP缺口末端标记（TUNEL）/神经元特异性核蛋白（NeuN）和5-溴-2'-脱氧尿苷（BrdU）/NeuN免疫荧光染色显示，与对照组相比，高氧组齿状颗粒下区凋亡细胞数和增殖细胞数显着增加。但在相同的高氧环境下，NR3A KO组的凋亡细胞数和增殖细胞数较高氧组明显减少。 NR3A KO组和高氧组观察到TUNEL+/NeuN+和BrdU+/NeuN+细胞。这些结果表明，NR3A 基因可以促进细胞凋亡，并介导因暴露于非生理性高浓度氧气而引起的发育中大脑的潜在损伤。
In the present study, 7 day postnatal C57/BL6 wild-type mice (hyperoxia group) and 7 day postnatal N-methyl-D-aspartate receptor subtype 3A knockout mice (NR3A KO group) were exposed to 75% oxygen and 15% nitrogen in a closed container for 5 days. Wild-type mice raised in normoxia served as controls. TdT-mediated dUTP nick end labeling (TUNEL)/neuron-specific nuclear protein (NeuN) and 5-bromo-2′-deoxyuridine (BrdU)/NeuN immunofluorescence staining showed that the number of apoptotic cells and the number of proliferative cells in the dentate subgranular zone significantly increased in the hyperoxia group compared with the control group. However, in the same hyperoxia environment, the number of apoptotic cells and the number of proliferative cells significantly decreased in the NR3A KO group compared with hyperoxia group. TUNEL+/NeuN+ and BrdU+/NeuN+ cells were observed in the NR3A KO and the hyperoxia groups. These results demonstrated that the NR3A gene can promote cell apoptosis and mediate the potential damage in the developing brain induced by exposure to non-physiologically high concentrations of oxygen.
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影响因子：
25
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影响因子：
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作者：
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影响因子：
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通讯作者：
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