Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches.
Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches.
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DOI：
10.1111/j.1582-4934.2009.00758.x
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发表时间：
2009-05
影响因子：
5.3
通讯作者：
Faussone-Pellegrini MS
中科院分区：
文献类型：
--
作者：
Popescu LM;Gherghiceanu M;Manole CG;Faussone-Pellegrini MS
近年来的研究表明，心外膜下存在多种细胞谱系，我们推测该区域可能存在心脏干细胞龛（CSCN）。用透射电镜观察，发现正常成年小鼠心外膜下至少有10种细胞共存：脂肪细胞、成纤维细胞、雪旺细胞和神经纤维、平滑肌细胞、肥大细胞、巨噬细胞、淋巴细胞、间质Cajal样细胞（ICLC）和心肌祖细胞（CMPs）。后者细胞位于冠状动脉和主动脉的起源区域，显示出非常不成熟或发育中的心肌细胞的典型特征。这些细胞中的一些相互连接，形成由基膜包围的柱状物，并嵌入由ICLC形成的细胞网络中。ICLC和CMP之间通过电子致密纳米结构或通过脱落囊泡发生复杂的细胞间通讯。本文首次报道了成年小鼠心肌中CSCN的超微结构，主要包括ICLC和CMP。不同发育阶段的驻留型CMP的存在证明心脏更新是一个连续的过程。我们认为，ICLC可能作为支持护士细胞的心脏龛，并可能负责激活，承诺和迁移的干细胞的龛。简而言之，不仅常驻心脏干细胞，而且ICLC调节肌细胞更新，并有助于损伤后的心脏细胞稳态和内源性修复/重塑。
Recent studies suggested that various cell lineages exist within the subepicardium and we supposed that this area could host cardiac stem cell niches (CSCNs). Using transmission electron microscopy, we have found at least 10 types of cells coexisting in the subepicardium of normal adult mice: adipocytes, fibroblasts, Schwann cells and nerve fibres, isolated smooth muscle cells, mast cells, macrophages, lymphocytes, interstitial Cajal-like cells (ICLCs) and cardiomyocytes progenitors (CMPs). The latter cells, sited in the area of origin of coronary arteries and aorta, showed typical features of either very immature or developing cardiomyocytes. Some of these cells were connected to each other to form columns surrounded by a basal lamina and embedded in a cellular network made by ICLCs. Complex intercellular communication occurs between the ICLCs and CMPs through electron-dense nanostructures or through shed vesicles. We provide here for the first time the ultrastructural description of CSCN in the adult mice myocardium, mainly containing ICLCs and CMPs. The existence of resident CMPs in different developmental stages proves that cardiac renewing is a continuous process. We suggest that ICLCs might act as supporting nurse cells of the cardiac niches and may be responsible for activation, commitment and migration of the stem cells out of the niches. Briefly, not only resident cardiac stem cells but also ICLCs regulate myocyte turnover and contribute to both cardiac cellular homeostasis and endogenous repair/remodelling after injuries.
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影响因子：
37.8
作者：
Anversa, P;Kajstura, J;Bolli, R
通讯作者：
Bolli, R
影响因子：
21.3
作者：
Gillette, Jennifer M.;Larochelle, Andre;Dunbar, Cynthia E.;Lippincott-Schwartz, Jennifer
通讯作者：
Lippincott-Schwartz, Jennifer
DOI：
10.1073/pnas.0808357105
发表时间：
2008-10-07
影响因子：
11.1
作者：
Boni, Alessandro;Urbanek, Konrad;Leri, Annarosa
通讯作者：
Leri, Annarosa
影响因子：
7.7
作者：
Dellatore, Shara M.;Garcia, A. Sofia;Miller, William M.
通讯作者：
Miller, William M.
影响因子：
2.2
作者：
Hare JM;Chaparro SV
通讯作者：
Chaparro SV