Protein separation by capillary gel electrophoresis: a review.
Protein separation by capillary gel electrophoresis: a review.
复制标题
DOI:
10.1016/j.aca.2011.10.022
复制
发表时间:
2012-01-04
影响因子:
6.2
通讯作者:
Liu, Shaorong
中科院分区:
文献类型:
--
作者:
Zhu, Zaifang;Lu, Joann J.;Liu, Shaorong
关键词:
Capillary gel electrophoresis (CGE) has been used for protein separation for more than two decades. Due to the technology advancement, current CGE methods are becoming more and more robust and reliable for protein analysis, and some of the methods have been routinely used for the analysis of protein-based pharmaceuticals and quality controls. In light of this progress, we survey 147 papers related to CGE separations of proteins and present an overview of this technology. We first introduce briefly the early development of CGE. We then review the methodology, in which we specifically describe the matrices, coatings, and detection strategies used in CGE. CGE using microfabricated channels and incorporation of CGE with two-dimensional protein separations are also discussed in this section. We finally present a few representative applications of CGE for separating proteins in real-world samples.
登录
查看更多内容
DOI:
10.1016/s0021-9673(01)85026-3
发表时间:
1987-06-26
期刊:
JOURNAL OF CHROMATOGRAPHY
影响因子:
--
作者:
COHEN, AS;KARGER, BL
通讯作者:
KARGER, BL
影响因子:
7.4
作者:
COBB, KA;DOLNIK, V;NOVOTNY, M
通讯作者:
NOVOTNY, M
影响因子:
4.1
作者:
BUSCH, MHA;KRAAK, JC;POPPE, H
通讯作者:
POPPE, H
影响因子:
1.6
作者:
Bautista, J;Mateos-Nevado, MD
通讯作者:
Mateos-Nevado, MD
影响因子:
2.9
作者:
Chiu, TC;Lin, YW;Chang, HT
通讯作者:
Chang, HT