Protein separation by capillary gel electrophoresis: a review.

Protein separation by capillary gel electrophoresis: a review.
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DOI:
10.1016/j.aca.2011.10.022
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发表时间:
2012-01-04
影响因子:
6.2
通讯作者:
Liu, Shaorong
Liu, Shaorong
中科院分区:
化学1区
文献类型:
--
作者:
Zhu, Zaifang;Lu, Joann J.;Liu, Shaorong

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毛细管凝胶电泳(CGE)用于蛋白质分离已有二十多年的历史。由于技术的进步,目前的CGE方法正变得越来越强大和可靠的蛋白质分析,和一些方法已被常规用于蛋白质类药物的分析和质量控制。鉴于这一进展,我们调查了147篇与蛋白质的CGE分离相关的论文,并对该技术进行了概述。本文首先简要介绍了CGE的早期发展。然后,我们审查的方法,其中我们具体描述的矩阵,涂层,和检测策略中使用的CGE。本节还讨论了使用微通道的CGE和CGE与二维蛋白质分离的结合。最后,我们提出了几个代表性的应用CGE分离蛋白质在现实世界中的样品。
Capillary gel electrophoresis (CGE) has been used for protein separation for more than two decades. Due to the technology advancement, current CGE methods are becoming more and more robust and reliable for protein analysis, and some of the methods have been routinely used for the analysis of protein-based pharmaceuticals and quality controls. In light of this progress, we survey 147 papers related to CGE separations of proteins and present an overview of this technology. We first introduce briefly the early development of CGE. We then review the methodology, in which we specifically describe the matrices, coatings, and detection strategies used in CGE. CGE using microfabricated channels and incorporation of CGE with two-dimensional protein separations are also discussed in this section. We finally present a few representative applications of CGE for separating proteins in real-world samples.
DOI: 10.1016/s0021-9673(01)85026-3
发表时间: 1987-06-26
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