Changes in urethral smooth muscle and external urethral sphincter function with age in rats
Changes in urethral smooth muscle and external urethral sphincter function with age in rats
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DOI：
10.14814/phy2.14643
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发表时间：
2020-12-23
影响因子：
2.5
通讯作者：
Miyazato M
中科院分区：
文献类型：
--
作者：
Oshiro T;Kimura R;Izumi K;Ashikari A;Saito S;Miyazato M
为了确认尿道活动随年龄的变化，记录膀胱内压和尿道灌注压（UPP），并进行尿道外括约肌肌电图（EUS-EMG）。共使用了33只3月龄（幼龄大鼠）、12月龄（中年大鼠）和24月龄（老年大鼠）雌性Sprague道利大鼠。采用连续膀胱测压法评价膀胱活动度。在尿烷麻醉下，在等容条件下，通过同时记录各组的膀胱内压和UPP来评估尿道活动。此外，在相同条件下监测EUS-EMG活动。在连续膀胱测压中，三组之间的膀胱收缩幅度无差异;然而，与年轻大鼠相比，中年和老年大鼠的残余尿量显著增加。关于UPP，老年大鼠（60%）和中年大鼠（64%）的UPP变化显著小于年轻大鼠。此外，老年大鼠（61%）和中年大鼠（70%）EUS高频振荡的平均振幅显著低于年轻大鼠。EUS-EMG显示年轻大鼠排尿期间EUS爆发活动，具有清晰的活动期和沉默期，但老年大鼠的活动期和沉默期不清楚。尿道Masson三色染色显示，与年轻和中年大鼠相比，老年大鼠的EUS萎缩。结果提示，年龄的增长导致尿道平滑肌和EUS两种功能障碍，可能导致膀胱与尿道的协调失调。为了确认尿道活动随年龄的变化，记录膀胱内压和尿道灌注压（UPP），并进行尿道外括约肌肌电图（EUS-EMG）。青年、中年和老年大鼠的膀胱收缩幅度无差异;然而，中年和老年大鼠的残余尿量显著增加，老年大鼠的UPP变化显著较小。在组织学上，老年大鼠EUS萎缩。结果提示，年龄的增长导致尿道平滑肌和EUS两种功能障碍，可能导致膀胱与尿道的协调失调。
To confirm changes in urethral activity with age, both intravesical pressure and urethral perfusion pressure (UPP) were recorded and external urethral sphincter electromyography (EUS‐EMG) was performed. A total of 33 female Sprague Dawley rats aged 3 months (young rats), 12 months (middle‐aged rats), and 24 months (aged rats) were used. Bladder activity was evaluated using continuous cystometry. Urethral activity was evaluated by simultaneously recording intravesical pressure and UPP in isovolumetric conditions under urethane anesthesia in each group. Additionally, EUS‐EMG activity was monitored under the same conditions. In continuous cystometry, the amplitude of bladder contractions was not different among the three groups; nevertheless, residual urine volume was significantly increased in middle‐aged and aged rats, as compared in young rats. With respect to UPP, the change in UPP was significantly smaller in aged rats (60%) and middle‐aged rats (64%) than in young rats. Furthermore, the mean amplitude of high‐frequency oscillations of the EUS was significantly lower in aged (61%) and middle‐aged rats (70%) than in young rats. EUS‐EMG revealed EUS bursting activity during voiding with clear active and silent phases in young rats but unclear active and silent phases in aged rats. Masson's trichrome staining of the urethra showed EUS atrophy in aged rats compared to young and middle‐aged rats. The results indicate that aging induces two urethral dysfunctions in the urethral smooth muscle and EUS, which may lead to dyscoordination between the urinary bladder and urethra. To confirm changes in urethral activity with age, both intravesical pressure and urethral perfusion pressure (UPP) were recorded and external urethral sphincter electromyography (EUS‐EMG) was performed. The amplitude of bladder contractions was not different among young, middle‐aged, and aged rats; nevertheless, residual urine volume was significantly increased in middle‐aged and aged rats and the change in UPP was significantly smaller in aged rats. In histological findings, EUS hypotrophy was observed in aged rats. The results indicate that aging induces two urethral dysfunctions in the urethral smooth muscle and EUS, which may lead to dyscoordination between the urinary bladder and urethra.
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影响因子：
4.4
作者：
Bachschmid MM;Schildknecht S;Matsui R;Zee R;Haeussler D;Cohen RA;Pimental D;Loo Bv
通讯作者：
Loo Bv
影响因子：
7.3
作者：
Rea IM;Gibson DS;McGilligan V;McNerlan SE;Alexander HD;Ross OA
通讯作者：
Ross OA
影响因子：
6.6
作者：
MORITA, T;NISHIZAWA, O;TSUCHIDA, S
通讯作者：
TSUCHIDA, S
影响因子：
6.1
作者：
Oshiro, Takuma;Miyazato, Minoru;Saito, Seiichi
通讯作者：
Saito, Seiichi
DOI：
10.1152/ajpregu.00270.2007
发表时间：
2008-02-01
影响因子：
2.8
作者：
Peng, Chih-Wei;Chen, Jia-Jin Jason;Grill, Warren M.
通讯作者：
Grill, Warren M.