Methamphetamine decreases CD4 T cell frequency and alters pro-inflammatory cytokine production in a model of drug abuse.
Methamphetamine decreases CD4 T cell frequency and alters pro-inflammatory cytokine production in a model of drug abuse.
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DOI：
10.1016/j.ejphar.2015.02.002
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发表时间：
2015-04-05
影响因子：
5
通讯作者：
Baum LL
中科院分区：
文献类型：
--
作者：
Mata MM;Napier TC;Graves SM;Mahmood F;Raeisi S;Baum LL
甲基苯丙胺的使用和艾滋病毒感染导致艾滋病进展更快的原因尚不清楚。我们使用甲基苯丙胺自我给药模型来测量甲基苯丙胺对系统免疫系统的影响，以更好地了解甲基苯丙胺和艾滋病毒的共病。将导管植入雄性SD大鼠的颈静脉，使其能够自我给药(n=18)或给予生理盐水(n=16)，持续14天。在最后一次自我给药后的一天，采集血液和脾。检测血清促炎细胞因子、细胞内干扰素-γ和肿瘤坏死因子-α水平，以及脾组织中CD_4~+、CD_8~+、CD_(200)~+和CD_(11)b/c~+淋巴细胞的比例。自我给予甲基苯丙胺的大鼠的CD+T细胞频率较低，但这些细胞中更多的产生干扰素-γ。甲基苯丙胺不会改变产生肿瘤坏死因子-α的T细胞的频率。甲基苯丙胺组大鼠外周血中CD8+T细胞比例较高，但产生肿瘤坏死因子-α的比例较低。CD11b/c和CD200表达无明显变化。甲基苯丙胺大鼠血清细胞因子干扰素-γ、肿瘤坏死因子-α和IL-6水平无明显变化。甲基苯丙胺终生剂量与血清肿瘤坏死因子-α水平呈负相关。或者数据表明，滥用甲基苯丙胺可能会通过激活CD4T细胞，使它们更容易受到艾滋病毒感染，并导致它们过早死亡，从而加剧艾滋病毒疾病的进展。甲基苯丙胺也可能增加对艾滋病毒感染的易感性，这解释了为什么与男性发生性行为(MSM)并经常使用甲基苯丙胺的非裔美国男性感染艾滋病毒的风险最高。
The reason co-morbid methamphetamine use and HIV infection lead to more rapid progression to AIDS is unclear. We used a model of methamphetamine self-administration to measure the effect of methamphetamine on the systemic immune system to better understand the comorbidity of methamphetamine and HIV. Catheters were implanted into the jugular veins of male, Sprague Dawley rats so they could self-administer methamphetamine (n = 18) or be given saline (control; n = 16) for 14 days. One day after the last self-administration session, blood and spleens were collected. We measured serum levels of pro-inflammatory cytokines, intracellular IFN-γand TNF-α, and frequencies of CD4+, CD8+, CD200+ and CD11b/c+ lymphocytes in the spleen. Rats that self-administer methamphetamine had a lower frequency of CD4+ T cells, but more of these cells produced IFN-γ. Methamphetamine did not alter the frequency of TNF-α-producing CD4+ T cells. Methamphetamine using rats had a higher frequency of CD8+ T cells, but fewer of them produced TNF-α. CD11b/c and CD200 expression were unchanged. Serum cytokine levels of IFN-γ, TNF-α and IL-6 in methamphetamine rats were unchanged. Methamphetamine lifetime dose inversely correlated with serum TNF-α levels. Or data suggest that methamphetamine abuse may exacerbate HIV disease progression by activating CD4 T cells, making them more susceptible to HIV infection, and contributing to their premature demise. Methamphetamine may also increase susceptibility to HIV infection, explaining why African American men who have sex with men (MSM) and frequently use methamphetamine are at the highest risk of HIV infection.
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影响因子：
3.7
作者：
Volkow ND;Fowler JS;Wang GJ;Shumay E;Telang F;Thanos PK;Alexoff D
通讯作者：
Alexoff D
DOI：
10.1097/01.qai.0000230530.02212.86
发表时间：
2006-11-01
影响因子：
3.6
作者：
Drumright, Lydia N.;Little, Susan J.;Gorbach, Pamina M.
通讯作者：
Gorbach, Pamina M.
影响因子：
6.2
作者：
Nair, Madhavan P. N.;Mahajan, Supriya;Reynolds, Jessica L.
通讯作者：
Reynolds, Jessica L.
影响因子：
3.1
作者：
Valencia, Frances;Bubar, Marcy J.;Bourne, Nigel
通讯作者：
Bourne, Nigel
Mirtazapine改变了在大鼠中寻求与提示相关的甲基苯丙胺。
影响因子：
10.6
作者：
Graves, Steven M.;Napier, T. Celeste
通讯作者：
Napier, T. Celeste