Contactless cell trapping by the use of a uniform AC electric field.
Contactless cell trapping by the use of a uniform AC electric field.
复制标题
使用均匀交流电场进行非接触式细胞捕获。
作者：
S. Tada;Tomoyuki Natsuya;A. Tsukamoto;Yudai Santo
交流电场驱动的悬浮极化粒子操控已成为微米和纳米器件的主要技术。在本研究中，使用等渗溶液中培养的HeLa细胞的悬浮液来探索在频率1 kHz、强度E(rms)=1.67×10(4) V/m的均匀交流电场下悬浮行为的机制。基于粒子动力学朗之万方程的分子动力学（MD）模拟阐明了相应的问题。新开发了一种计算悬浮介质中机电、粘性和重力作用下单个细胞轨迹的理论模型。数值计算表明，在现场应用的早期阶段，悬浮细胞开始沿着均匀交流电场的方向聚集形成链状簇。此外，预测结果与实验结果相似。这些发现表明，链状细胞簇是由相邻细胞的长程偶极子相互作用产生的，但在重力的作用下，可能阻碍簇在垂直方向上的生长。
The AC electric field-driven manipulation of suspended polarizable particles has become a major technique in micro- and nano-devices. In the present study, suspensions of cultured HeLa cells in isotonic solution were used to explore the mechanisms underlying the suspension behaviors during exposure to a uniform AC electric field of strength E(rms)=1.67×10(4) V/m at frequency 1 kHz. Molecular dynamics (MD) simulations based on the Langevin equation of particle kinetics were performed to elucidate the corresponding problem. A theoretical model to compute the trajectories of individual cells under the action of electro-mechanical, viscous and gravitational forces in the suspending medium was newly developed. Numerical computations demonstrated that the suspended cells began to aggregate to form chainlike clusters along the direction of the uniform AC electric field at an earlier stage of the field application. Moreover, the predicted results were similar to the experimental results. These findings indicate that the chain-like cell clustering arises from the long-range dipole-dipole interaction of neighboring cells, but under the action of the gravitational force that likely hinders the growth of clusters in the vertical direction.
影响因子：
7.4
作者：
Das, CM;Becker, F;Gascoyne, PRC
通讯作者：
Gascoyne, PRC
影响因子：
6.2
作者：
Satori CP;Kostal V;Arriaga EA
通讯作者：
Arriaga EA