Review on recent advances in the analysis of isolated organelles.

Review on recent advances in the analysis of isolated organelles.
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DOI:
10.1016/j.aca.2012.09.041
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发表时间:
2012-11-13
影响因子:
6.2
通讯作者:
Arriaga EA
Arriaga EA
中科院分区:
化学1区
文献类型:
--
作者:
Satori CP;Kostal V;Arriaga EA

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The analysis of isolated organelles is one of the pillars of modern bioanalytical chemistry. This review describes recent developments on the isolation and characterization of isolated organelles both from living organisms and cell cultures. Salient reports on methods to release organelles focused on reproducibility and yield, membrane isolation, and integrated devices for organelle release. New developments on organelle fractionation after their isolation were on the topics of centrifugation, immunocapture, free flow electrophoresis, flow field-flow fractionation, fluorescence activated organelle sorting, laser capture microdissection, and dielectrophoresis. New concepts on characterization of isolated organelles included atomic force microscopy, optical tweezers combined with Raman spectroscopy, organelle sensors, flow cytometry, capillary electrophoresis, and microfluidic devices.
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