Identification and characterisation of eroA and ervA, encoding two putative thiol oxidases from Aspergillus niger.
Identification and characterisation of eroA and ervA, encoding two putative thiol oxidases from Aspergillus niger.
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eroA 和 ervA 的鉴定和表征，编码两种假定的黑曲霉硫醇氧化酶。
DOI：
10.1016/j.gene.2010.04.011
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发表时间：
2010
期刊：
影响因子：
3.5
通讯作者：
Harvey AR
中科院分区：
文献类型：
--
作者：
Harvey AR
蛋白质在分泌途径中的氧化折叠涉及二硫键的形成和异构化，并由内质网(ER)管腔中的折叠酶催化。从二硫键的形成到氧的还原当量的转移涉及硫醇氧化酶的参与。在这里，我们描述了来自黑曲霉的eroA和erva基因的鉴定和功能特性，它们分别编码酿酒酵母ERO1和ERV2的功能同源物。EroA基因编码600个氨基酸的产物EroA，erva基因编码215个氨基酸的产物Erva，两者与酿酒酵母的同源物有共同的基序和特征。与酿酒酵母中的Ero1p不同，黑曲霉的EroA似乎通过C末端的保留基序保留在内质网管腔中。实时荧光定量PCR分析表明，ERA在内质网应激时转录上调，而Erva在DTT胁迫下略有下调，但在异源蛋白表达时上调。基因破坏研究表明，与Erva不同，eroA是生存所必需的。当在温度敏感的酿酒酵母ero1-1中表达时，eroA和erva都能够补充温度和DTT敏感的表型，尽管截短的eroA不能补充全长的eroA基因和全长的eroA基因。
The oxidative folding of proteins in the secretory pathway involves the formation and isomerisation of disulphide bonds and is catalysed by foldases in the lumen of the endoplasmic reticulum (ER). The transfer of reducing equivalents, from disulphide bond formation, to oxygen involves the participation of thiol oxidases. Here, we describe the identification and functional characterisation of the eroA and ervA genes from Aspergillus niger, encoding functional orthologues of S. cerevisiae ERO1 and ERV2, respectively. The eroA gene encodes a product of 600 amino acids, EroA, and the ervA gene encodes a product of 215 amino acids, ErvA, both of which share common motifs and features with their S. cerevisiae orthologues. In contrast to Ero1p in S. cerevisiae, A. niger EroA appears to be retained in the ER lumen by a C-terminal retention motif. Real-time PCR analysis indicated that eroA is transcriptionally up-regulated in response to ER stress, whereas ervA is slightly down-regulated in response to DTT stress yet up-regulated in response to expression of a heterologous protein. Gene disruption studies indicated that, unlike ervA, eroA is essential for viability. When expressed in the thermosensitive S. cerevisiae ero1-1 strain, both eroA and ervA were able to complement the temperature and DTT sensitive phenotype, although a truncated eroA, missing the putative HEEL ER-retention signal was unable to complement as well as the full-length eroA gene.
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编码黑曲霉 bipA 基因的 ER 伴侣由热休克和未折叠蛋白诱导。
影响因子：
3.5
作者：
I. A. van Gemeren;P. Punt;A. Drint;M.P Broekhuijsen;A. van 't Hoog;A. Beijersbergen;C. T. Verrips;C. A. van den Hondel
通讯作者：
C. A. van den Hondel
DOI：
10.1038/nsb740
发表时间：
2002-01-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Gross, E;Sevier, CS;Fass, D
通讯作者：
Fass, D
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影响因子：
1.5
作者：
P. Melin;M. Stratford;A. Plumridge;D. Archer
通讯作者：
D. Archer
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影响因子：
1.6
作者：
M. Elder;R. Montgomery
通讯作者：
R. Montgomery
影响因子：
46.9
作者：
Pel, Herman J.;de Winde, Johannes H.;Stam, Hein
通讯作者：
Stam, Hein