Chemical Analysis of Morphological Changes in Lysophosphatidic Acid-Treated Ovarian Cancer Cells.
Chemical Analysis of Morphological Changes in Lysophosphatidic Acid-Treated Ovarian Cancer Cells.
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DOI：
10.1038/s41598-017-15547-7
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发表时间：
2017-11-10
影响因子：
4.6
通讯作者：
Schultz ZD
中科院分区：
文献类型：
--
作者：
Bailey KA;Klymenko Y;Feist PE;Hummon AB;Stack MS;Schultz ZD
据报道，卵巢癌(OvCa)细胞在溶血磷脂酸(LPA)处理后，细胞表面发生生化变化。在这里，我们使用扫描电子显微镜(SEM)和超连续谱激发下的多重相干反斯托克斯拉曼散射(CARS)成像来探测LPA处理后的形态变化。扫描电子显微镜图像显示在LPA处理后，微绒毛样特征明显脱落。对多重CARS图像的分析可以区分分子成分，如脂类和蛋白质。我们的结果表明，OvCa429和SKOV3ip上皮性卵巢癌细胞对LPA的处理具有相似的形态和化学反应。经LPA处理后，多细胞聚集体(MCAS)表面的微绒毛样结构被去除。CARS分析表明，LPA处理的细胞表面蛋白质明显减少，脂肪成分增加。重要的是，来自LPA处理的MCAS细胞脱落的CARS信号与最初存在的蛋白质的切割是一致的。对细胞脱落的质谱分析表明，细胞内和膜上都存在大量的蛋白质。与上皮细胞相比，间充质细胞系的多肽数量增加，这表明随着癌症的进展，对LPA治疗的反应不同。
Ovarian cancer (OvCa) cells are reported to undergo biochemical changes at the cell surface in response to treatment with lysophosphatidic acid (LPA). Here we use scanning electron microscopy (SEM) and multiplex coherent anti-Stokes Raman scattering (CARS) imaging via supercontinuum excitation to probe morphological changes that result from LPA treatment. SEM images show distinct shedding of microvilli-like features upon treatment with LPA. Analysis of multiplex CARS images can distinguish between molecular components, such as lipids and proteins. Our results indicate that OvCa429 and SKOV3ip epithelial ovarian cancer cells undergo similar morphological and chemical responses to treatment with LPA. The microvilli-like structures on the surface of multicellular aggregates (MCAs) are removed by treatment with LPA. The CARS analysis shows a distinct decrease in protein and increase in lipid composition on the surface of LPA-treated cells. Importantly, the CARS signals from cellular sheddings from MCAs with LPA treatment are consistent with cleavage of proteins originally present. Mass spectrometry on the cellular sheddings show that a large number of proteins, both membrane and intracellular, are present. An increased number of peptides are detected for the mesenchymal cell line relative to the epithelial cell indicating a differential response to LPA treatment with cancer progression.
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影响因子：
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