Nitric oxide functions as a signal in ultraviolet-B-induced baicalin accumulation in Scutellaria baicalensis suspension cultures.
Nitric oxide functions as a signal in ultraviolet-B-induced baicalin accumulation in Scutellaria baicalensis suspension cultures.
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一氧化氮作为黄芩悬浮培养物中紫外线 B 诱导的黄芩苷积累的信号
DOI：
10.3390/ijms15034733
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发表时间：
2014-03-18
影响因子：
5.6
通讯作者：
Jin SH
中科院分区：
文献类型：
--
作者：
Zhang JJ;Li XQ;Sun JW;Jin SH
紫外线B(UV-B)辐射诱导的胁迫刺激植物体内各种次生代谢物的积累。一氧化氮(NO)在UV-B应激诱导的信号转导通路中起着重要的辅助信使作用。在植物中，NO可以通过酶催化或无机氮途径合成。UV-B辐射对植物细胞中黄芩苷产生的影响及其相关的分子途径还知之甚少。研究了UV-B辐射对黄芩细胞悬浮培养中一氧化氮合酶(NOS)活性、NO释放量和黄芩苷生成的影响。UV-B辐射显著增加黄褐斑病菌细胞内一氧化氮合酶活性、NO释放和黄芩苷的生物合成。此外，由NO供体硝普钠(SNP)提供的外源NO导致了与UV-B处理相似的黄芩苷含量的增加。一氧化氮清除剂2-(4-carboxyphenyl)-4，4，5，5-tetramethylimidazoline-1-oxyl-3-oxide(CPTIO)和一氧化氮合酶抑制剂N-ω-硝基-L-精氨酸(LNNA)部分抑制UV-B诱导的NO释放和黄芩苷的积累。这些结果表明，NO是由一氧化氮合酶或类一氧化氮合酶产生的，在黄芩苷的生物合成中起着重要的作用，这是黄芩苷细胞对UV-B辐射的防御反应的一部分。
Stress induced by ultraviolet-B (UV-B) irradiation stimulates the accumulation of various secondary metabolites in plants. Nitric oxide (NO) serves as an important secondary messenger in UV-B stress-induced signal transduction pathways. NO can be synthesized in plants by either enzymatic catalysis or an inorganic nitrogen pathway. The effects of UV-B irradiation on the production of baicalin and the associated molecular pathways in plant cells are poorly understood. In this study, nitric oxide synthase (NOS) activity, NO release and the generation of baicalin were investigated in cell suspension cultures of Scutellaria baicalensis exposed to UV-B irradiation. UV-B irradiation significantly increased NOS activity, NO release and baicalin biosynthesis in S. baicalensis cells. Additionally, exogenous NO supplied by the NO donor, sodium nitroprusside (SNP), led to a similar increase in the baicalin content as the UV-B treatment. The NOS inhibitor, Nω-nitro-l-arginine (LNNA), and NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) partially inhibited UV-B-induced NO release and baicalin accumulation. These results suggest that NO is generated by NOS or NOS-like enzymes and plays an important role in baicalin biosynthesis as part of the defense response of S. baicalensis cells to UV-B irradiation.
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影响因子：
4.8
作者：
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DOI：
10.1016/j.jphotobiol.2011.05.004
发表时间：
2011-09-02
影响因子：
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