Construction of an efficient Claviceps paspali cell factory for lysergic acid production.
Construction of an efficient Claviceps paspali cell factory for lysergic acid production.
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DOI：
10.3389/fbioe.2022.1093402
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发表时间：
2022
影响因子：
5.7
通讯作者：
Lu, Xuefeng
中科院分区：
文献类型：
--
作者：
Hu, Mingzhe;Zhou, Yu;Du, Siyu;Zhang, Xuan;Tang, Shen;Yang, Yong;Zhang, Wei;Chen, Shaoxin;Huang, Xuenian;Lu, Xuefeng
Lysergic acid (LA) is the key precursor of ergot alkaloids, and its derivatives have been used extensively for the treatment of neurological disorders. However, the poor fermentation efficiency limited its industrial application. At the same time, the hardship of genetic manipulation has hindered the metabolic engineering of Claviceps strains to improve the LA titer further. In this study, an efficient genetic manipulation system based on the protoplast-mediated transformation was established in the industrial strain Claviceps paspali. On this basis, the gene lpsB located in the ergot alkaloids biosynthetic gene cluster was deleted to construct the LA-producing cell factory. Plackett-Burman and Box-Behnken designs were used in shaking flasks, achieving an optimal fermentation medium composition. The final titer of LA and iso-lysergic acid (ILA) reached 3.7 g·L−1, which was 4.6 times higher than that in the initial medium. Our work provides an efficient strategy for the biosynthesis of LA and ILA and lays the groundwork for its industrial production.
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影响因子：
6.4
作者：
Nielsen CA;Folly C;Hatsch A;Molt A;Schröder H;O'Connor SE;Naesby M
通讯作者：
Naesby M
影响因子：
4.6
作者：
Abuhena, Md;Al-Rashid, Jubair;Azim, Md Faisal;Khan, Md Niuz Morshed;Kabir, Md Golam;Barman, Nirmal Chandra;Rasul, Noorain Munim;Akter, Shahina;Huq, Md Amdadul
通讯作者：
Huq, Md Amdadul
影响因子：
4.4
作者：
Arnold, Stephanie L.;Panaccione, Daniel G.
通讯作者：
Panaccione, Daniel G.
影响因子：
5.2
作者：
Liu, Qiang;Jia, Yanxing
通讯作者：
Jia, Yanxing
影响因子：
5
作者：
Kozák L;Szilágyi Z;Vágó B;Kakuk A;Tóth L;Molnár I;Pócsi I
通讯作者：
Pócsi I