Optimization of industrial (3000 L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation.
Optimization of industrial (3000 L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation.
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枯草芽孢杆菌CW-S工业化生产条件的优化及其在微型块茎和工业化马铃薯栽培中的新应用。
DOI：
10.1038/s41598-022-15366-5
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发表时间：
2022-07-01
影响因子：
4.6
通讯作者：
Huq, Md Amdadul
中科院分区：
文献类型：
--
作者：
Abuhena, Md;Al-Rashid, Jubair;Azim, Md Faisal;Khan, Md Niuz Morshed;Kabir, Md Golam;Barman, Nirmal Chandra;Rasul, Noorain Munim;Akter, Shahina;Huq, Md Amdadul
利用枯草芽孢杆菌CW-S深层发酵生产植物益生菌商品化产品。以低成本生产为目标，研究了糖蜜和尿素对细胞生长的影响。采用Plackett-Burman法和中心复合设计法对生产参数进行优化，使生产效率最大化。对配方产品的稳定性进行了评价，并对其在气培微型块茎和工业级马铃薯大田栽培中的效果进行了评价。结果表明，BS10(糖蜜和尿素)的菌体密度(7.19 × 108cfu/m L)显著高于对照(1.51 × 107cfu/m L)和BS1-BS9(昂贵)培养基(1.84 × 107-1.37 × 109cfu/m L)。根据验证的ccd结果，优化后的参数在中试(300cfu L；2.05cfu× 109cfu/mL)和工业(3000cfu L；2.01cfu× 109cfu/m L)生物反应器中拟合良好，使细胞浓度比实验室(9.84cfu× 108cfu/m L)提高了一倍。在气培方面，CW-S取得了很好的效果，显著增加了微型薯的数量和重量，提高了移栽成活率。在田间试验中，工业级(>； 55 mm)马铃薯的产量随着化肥用量的减少而增加。总体而言，研究结果表明，CW-S可以利用新开发的培养基和优化的条件进行商业化生产，使植物益生菌更具成本效益，农民可以用于作物种植，特别是在气培微型块茎和工业级马铃薯生产中。
A commercial plant probiotic product was developed employing Bacillus subtilis CW-S in submerged fermentation. The effects of molasses and urea on cell growth were investigated with the goal of low-cost manufacturing. Plackett–Burman and Central-Composite Design (CCD) were utilized to optimize production parameters to maximize productivity. The stability of the formulated product and its efficacy in cultivating minituber in aeroponics and industrial-grade potatoes in the field were assessed. The results showed that the medium BS10 (molasses and urea) produced satisfactory cell density (7.19 × 108 CFU/mL) as compared to the control (1.51 × 107 CFU/mL) and BS1-BS9 (expensive) media (1.84 × 107–1.37 × 109 CFU/mL). According to validated CCD results, optimized parameters fitted well in pilot (300 L; 2.05 × 109 CFU/mL) and industrial (3000 L; 2.01 × 109 CFU/mL) bioreactors, resulting in a two-fold increase in cell concentration over laboratory (9.84 × 108 CFU/mL) bioreactors. In aeroponics, CW-S produced excellent results, with a significant increase in the quantity and weight of minitubers and the survival rate of transplanted plantlets. In a field test, the yield of industrial-grade (> 55 mm) potatoes was increased with a reduction in fertilizer dose. Overall, the findings suggest that CW-S can be produced commercially utilizing the newly developed media and optimized conditions, making plant probiotics more cost-effective and accessible to farmers for crop cultivation, particularly in aeroponic minituber and industrial-grade potato production.
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影响因子：
2.4
作者：
Chaudhari, Suraj R.;Shirkhedkar, Atul A.
通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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