Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.
Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.
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结构照明显微镜下神经元结构的超分辨率成像。
DOI:
10.3390/bioengineering10091081
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发表时间:
2023-09-13
影响因子:
4.6
通讯作者:
Hagen, Guy M.
中科院分区:
文献类型:
--
作者:
Paul, Tristan C.;Johnson, Karl A.;Hagen, Guy M.
Super-resolution structured illumination microscopy (SR-SIM) is an optical fluorescence microscopy method which is suitable for imaging a wide variety of cells and tissues in biological and biomedical research. Typically, SIM methods use high spatial frequency illumination patterns generated by laser interference. This approach provides high resolution but is limited to thin samples such as cultured cells. Using a different strategy for processing raw data and coarser illumination patterns, we imaged through a 150-micrometer-thick coronal section of a mouse brain expressing GFP in a subset of neurons. The resolution reached 144 nm, an improvement of 1.7-fold beyond conventional widefield imaging.
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影响因子:
48
作者:
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DOI:
10.1073/pnas.1609278114
发表时间:
2017-05-09
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通讯作者:
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