Satellite Cells Exhibit Decreased Numbers and Impaired Functions on Single Myofibers Isolated from Vitamin B6-Deficient Mice.
Satellite Cells Exhibit Decreased Numbers and Impaired Functions on Single Myofibers Isolated from Vitamin B6-Deficient Mice.
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DOI：
10.3390/nu13124531
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发表时间：
2021-12-17
期刊：
影响因子：
5.9
通讯作者：
Kumrungsee T
中科院分区：
文献类型：
--
作者：
Komaru T;Yanaka N;Kumrungsee T
最新的人体研究表明维生素B6、骨质疏松症和肌肉力量之间存在关联。然而，关于它在细胞水平上的潜在作用，特别是在肌肉卫星细胞中的潜在作用，人们知之甚少。因此，为了确定维生素B6是否影响卫星细胞，我们从维生素B6缺乏和补充维生素B6的小鼠的肌肉中分离出单个肌纤维。随后，我们对它们进行单个肌纤维培养，并观察卫星细胞的数量和功能，这些细胞留在肌纤维上的壁龛中。在培养之前，与补充维生素B6的肌纤维相比，缺乏维生素B6的肌纤维显示出明显较少的静止卫星细胞数量，从而表明缺乏维生素B6导致小鼠肌肉中静止的卫星细胞池的减少。培养48h和72h后，缺乏维生素B6和补充维生素B6的肌纤维中每簇增殖的卫星细胞数量相似，但在不含维生素B6的培养液中培养后卫星细胞数量显著减少。培养72小时后，缺乏维生素B6的肌纤维中每个簇的自我更新卫星细胞的数量明显减少，而不含维生素B6的培养液进一步减少了这一数量。综上所述，维生素B6缺乏似乎减少了静止的卫星细胞的数量，抑制了卫星细胞在肌肉发生过程中的增殖和自我更新。
Emerging research in human studies suggests an association among vitamin B6, sarcopenia, and muscle strength. However, very little is known regarding its potential role at the cellular level, especially in muscle satellite cells. Therefore, to determine whether vitamin B6 affects the satellite cells, we isolated single myofibers from muscles of vitamin B6-deficient and vitamin B6-supplemented mice. Subsequently, we subjected them to single myofiber culture and observed the number and function of the satellite cells, which remained in their niche on the myofibers. Prior to culture, the vitamin B6-deficient myofibers exhibited a significantly lower number of quiescent satellite cells, as compared to that in the vitamin B6-supplemented myofibers, thereby suggesting that vitamin B6 deficiency induces a decline in the quiescent satellite cell pool in mouse muscles. After 48 and 72 h of culture, the number of proliferating satellite cells per cluster was similar between the vitamin B6-deficient and -supplemented myofibers, but their numbers decreased significantly after culturing the myofibers in vitamin B6-free medium. After 72 h of culture, the number of self-renewing satellite cells per cluster was significantly lower in the vitamin B6-deficient myofibers, and the vitamin B6-free medium further decreased this number. In conclusion, vitamin B6 deficiency appears to reduce the number of quiescent satellite cells and suppress the proliferation and self-renewal of satellite cells during myogenesis.
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DOI：
10.31744/einstein_journal/2020ao5075
发表时间：
2020-01-01
期刊：
Einstein (São Paulo)
影响因子：
--
作者：
Viana, Ana Carolina Cavalcante;Aguiar, Ana Patrícia Nogueira;Maia, Fernanda Maria Machado
通讯作者：
Maia, Fernanda Maria Machado
DOI：
10.1093/gerona/62.4.434
发表时间：
2007-04-01
影响因子：
5.1
作者：
Kuo, Hsu-Ko;Liao, Kuo-Chen;Tail, Tong-Yuan
通讯作者：
Tail, Tong-Yuan
DOI：
10.1038/nrrheum.2017.60
发表时间：
2017-06
期刊：
Nature reviews. Rheumatology
影响因子：
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作者：
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通讯作者：
Cooper C
影响因子：
82.9
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影响因子：
64.8
作者：
Chakkalakal JV;Jones KM;Basson MA;Brack AS
通讯作者：
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