Identification and characterization of piperine synthase from black pepper, Piper nigrum L.
Identification and characterization of piperine synthase from black pepper, Piper nigrum L.
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DOI：
10.1038/s42003-021-01967-9
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发表时间：
2021-04-08
影响因子：
5.9
通讯作者：
Vogt T
中科院分区：
文献类型：
--
作者：
Schnabel A;Athmer B;Manke K;Schumacher F;Cotinguiba F;Vogt T
黑胡椒（Piper nigrum L.）是世界上最受欢迎的香料，也被用作传统药物的成分。其刺激性感觉是由于其主要化合物胡椒碱（1-胡椒酰-哌啶）与人TRPV-1或香草素受体的相互作用。我们现在确定了迄今为止隐藏的胡椒碱的酶促形成胡椒酰辅酶A和哌啶的基础上，从发展中的黑胡椒果实的差异RNA-Seq方法。这种酶被描述为胡椒碱合酶（胡椒酰辅酶A：哌啶胡椒酰转移酶），是BAHD型酰基转移酶的成员，由优先在未成熟果实中表达的基因编码。第二BAHD型酶，也高度表达在未成熟的黑胡椒果实，具有相当混杂的底物特异性，结合不同的CoA酯与脂肪族和芳香族胺具有类似的效率，并被称为胡椒酰胺合酶。重组胡椒碱和胡椒酰胺脱氢酶是黑胡椒中一个小基因家族的成员。它们可用于促进基于广泛的CoA供体和胺衍生受体的广泛的医学相关的脂肪族和芳香族哌啶酰胺的微生物生产，提供广泛的应用。Schnabel等人鉴定并表征了发育中的黑胡椒果实中的胡椒碱合酶，其催化由胡椒酰辅酶A和哌啶形成胡椒碱。BAHD型酰基转移酶的成员，这种酶可用于生物生产广泛的医药相关的哌酰胺。
Black pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, piperine (1-piperoyl-piperidine) with the human TRPV-1 or vanilloid receptor. We now identify the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits, has a rather promiscuous substrate specificity, combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic piperamides based on a wide array of CoA-donors and amine-derived acceptors, offering widespread applications. Schnabel et al. identify and characterize piperine synthase in developing black pepper fruits which catalyses the formation of piperine from piperoyl coenzyme A and piperidine. A member of BAHD-type acyltransferases, this enzyme can be useful for bio-production of a broad range of medicinally relevant piperamides.
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