Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.
Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.
复制标题
DOI：
10.1186/1471-2164-3-5
复制
发表时间：
2002
期刊：
影响因子：
4.4
通讯作者：
Korneluk RG
中科院分区：
文献类型：
--
作者：
Holcik M;Lefebvre CA;Hicks K;Korneluk RG
细胞凋亡抑制因子（Inhibitor of apoptosis，IAP）蛋白是细胞凋亡的重要内在调节因子。我们分离了大鼠细胞凋亡抑制基因1、2和3，并表征了它们的组织分布和表达。大鼠iap-1编码67.1 kDa的蛋白质，与人和小鼠iap-1的同源性分别为73%和89.2%.大鼠iap-2编码66.7kDa的蛋白质，与人和小鼠iap-2的同源性分别为81.6%和89.3%。大鼠iap-3编码56.1 kDa的蛋白质，与人和小鼠iap-3的同源性分别为89.5%和93.1%。我们已经产生了针对所有三个大鼠IAP基因的兔多克隆抗体。北方和Western印迹分析检测到大鼠IAP转录本和蛋白在大多数组织检查。此外，在睾丸中发现了一个与iap-2相对应的较短的可变剪接转录本。我们已经确定了三个大鼠同源的IAP基因。iap-1和iap-2在大鼠睾丸中的高表达提示这两个基因在精子发生中起重要的抗凋亡作用。
Inhibitor of Apoptosis (IAP) proteins are key intrinsic regulators of apoptosis induced by a variety of triggers. We isolated the rat Inhibitor of Apoptosis genes 1, 2 and 3 and characterized their tissue distribution and expression. Rat iap-1 encodes a protein of 67.1 kDa with 73 % and 89.2 % homology to human and mouse iap-1 respectively. Rat iap-2 encodes a protein of 66.7 kDa with 81.6 % and 89.3 % homology to human and mouse iap-2 respectively. Rat iap-3 encodes a protein of 56.1 kDa with 89.5 % and 93.1 % homology to human and mouse iap-3 respectively. We have generated rabbit polyclonal antibodies against all three rat IAP genes. Northern and Western blot analysis detected rat IAP transcripts and proteins in majority of the tissues examined. In addition, a shorter, alternatively spliced transcript corresponding to iap-2 was found in testes. We have identified three rat homologues of the IAP genes. The elevated expression of rat iap-1 and iap2 in testes suggests that these two genes play an important antiapoptotic role in spermatogenesis.
登录
查看更多内容
影响因子：
3.5
作者：
Korhonen, L;Belluardo, N;Lindholm, D
通讯作者：
Lindholm, D
影响因子：
4.4
作者：
Lagacé, M;Xuan, JY;Korneluk, RG
通讯作者：
Korneluk, RG
影响因子：
11.4
作者：
Deveraux, QL;Roy, N;Reed, JC
通讯作者：
Reed, JC
影响因子：
64.8
作者：
Liston, P;Roy, N;Korneluk, RG
通讯作者：
Korneluk, RG
影响因子：
4.4
作者：
Liston, P;Lefebvre, C;Korneluk, RG
通讯作者：
Korneluk, RG