Making the cut with PAMless CRISPR-Cas enzymes.
Making the cut with PAMless CRISPR-Cas enzymes.
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DOI:
10.1016/j.tig.2021.09.002
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发表时间:
2021-12
影响因子:
11.4
通讯作者:
Kleinstiver, Benjamin P.
中科院分区:
文献类型:
--
作者:
Christie, Kathleen A.;Kleinstiver, Benjamin P.
Genome editing technologies simplify our ability to rewrite genetic blueprints of life. However, CRISPR-Cas enzymes found in nature can only manipulate a fraction of the genome. Recently, new Cas variants have been developed that overcome this limitation, unlocking nearly the entire genome for editing.
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