Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation.
Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation.
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DOI：
10.1007/s00404-011-2141-1
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发表时间：
2012-05
影响因子：
2.6
通讯作者：
Choi JS
中科院分区：
文献类型：
--
作者：
Kang AR;Oh YR;Kim HY;Park MJ;Joo BS;Choi WJ;Lee JY;Jung MH;Ji YI;Choi JS
To investigate the effect of alendronate on the expression of Id genes in osteoblast differentiation. C2C12 cells were treated with alendronate for various concentrations and time periods. For evaluation of alendronate-induced osteoblast differentiation in C2C12 cells, alkaline phosphatase (ALP) activity was measured. The expression of osteoblast differentiation markers such as ALP, type-1 collagen (Col 1), and osteocalcin (OCN), and the expression of Id-1 and Id-2 were measured by RT-PCR. In order to understand the mechanism underlying the regulation of Id genes, the promoter region of the Id-1 gene was identified. Database analysis of the promoter region for Id-1 using known consensus sequences identified several putative response elements, including CCAAT/enhancer-binding protein beta (C/EBPβ). Alendronate treatment significantly increased not only ALP activity but also the expression of ALP, Col 1, and OCN, Id-1 and Id-2. C/EBPβ and alendronate cooperatively increased the promoter activity and expression of Id-1. These results suggest that C/EBPβ-mediated Id-1 transcriptional activation may regulate alendronate-induced osteoblast differentiation of C2C12 cells.
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影响因子：
4.8
作者：
Nakashima, A;Katagiri, T;Tamura, M
通讯作者：
Tamura, M
影响因子：
15.9
作者：
Plotkin, LI;Weinstein, RS;Bellido, T
通讯作者：
Bellido, T
影响因子：
3.3
作者：
Tominaga, Hiroyuki;Maeda, Shingo;Imamura, Takeshi
通讯作者：
Imamura, Takeshi
DOI：
10.1126/stke.2002.151.pe40
发表时间：
2002-09-24
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
--
作者：
Miyazono, Kohei;Miyazawa, Keiji
通讯作者：
Miyazawa, Keiji
阿仑膦酸钠通过 ERK 和 JNK 激活刺激骨髓基质细胞成骨分化并抑制成脂分化
影响因子：
4.1
作者：
Fu, Lingjie;Tang, Tingting;Dai, Kerong
通讯作者：
Dai, Kerong