Degradation and biocompatibility of multistage nanovectors in physiological systems.
Degradation and biocompatibility of multistage nanovectors in physiological systems.
复制标题
生理系统中多阶段纳米分离器的降解和生物相容性。
DOI：
10.1002/jbm.a.35017
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发表时间：
2014-10
影响因子：
4.9
通讯作者：
Tasciotti, Ennio
中科院分区：
文献类型：
--
作者：
Martinez, Jonathan O.;Evangelopoulos, Michael;Chiappini, Ciro;Liu, Xuewu;Ferrari, Mauro;Tasciotti, Ennio
The careful scrutiny of drug delivery systems is essential to evaluate and justify their potential for the clinic. Among the various studies necessary for pre-clinical testing, the impact of degradation is commonly overlooked. In this paper, we investigate the effect of fabrication (porosity and nucleation layer) and environment (buffer and pH) factors on the degradation kinetics of multi-stage nanovectors (MSV) composed of porous silicon. The degradation by-products of MSV were exposed to endothelial cells and analyzed for detrimental effects on cellular internalization, architecture, proliferation, and cell cycle. Increases in porosity resulted in accelerated degradation exhibiting smaller sized particles at comparable times. Removal of the nucleation layer (thin layer of small pores formed during the initial steps of etching) triggered a premature collapse of the entire central porous region of MSV. Variations in buffers prompted a faster degradation rate yielding smaller MSV within faster time frames while increases in pH stimulated erosion of MSV and thus faster degradation. In addition, exposure to these degradation by-products provoked negligible impact on the proliferation and cell cycle phases on primary endothelial cells. Here, we propose methods that lay the foundation for future investigations towards understanding the impact of the degradation of drug delivery platforms.
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影响因子：
38.3
作者：
通讯作者：
--
影响因子：
56.9
作者：
CARLISLE, EM
通讯作者：
CARLISLE, EM
影响因子：
14
作者：
Martinez, Jonathan O.;Chiappini, Ciro;Tasciotti, Ennio
通讯作者：
Tasciotti, Ennio
影响因子：
29.4
作者：
Canham, LT
通讯作者：
Canham, LT
DOI：
10.1002/pssa.200461134
发表时间：
2005-06-01
影响因子：
2
作者：
Coffer, JL;Whitehead, MA;Canham, LT
通讯作者：
Canham, LT