Acute cell viability and nitric oxide release in lateral menisci following closed-joint knee injury in a lapine model of post-traumatic osteoarthritis.
Acute cell viability and nitric oxide release in lateral menisci following closed-joint knee injury in a lapine model of post-traumatic osteoarthritis.
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DOI：
10.1186/1471-2474-15-297
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发表时间：
2014-09-06
影响因子：
2.3
通讯作者：
Haut Donahue TL
中科院分区：
文献类型：
--
作者：
Killian ML;Haut RC;Haut Donahue TL
外伤性嵌塞在体外可导致急性细胞死亡和软骨和半月板的肉眼损伤。本研究的目的是利用创伤负荷的原位模型研究内侧和外侧半月板的细胞活性和宏观损伤。此外，还记录了半月板、滑膜、软骨和软骨下骨中一氧化氮的释放。对5只兔左肢进行胫股嵌塞，造成前交叉韧带(ACL)断裂和半月板损伤。损伤后立即评估半月板撕裂形态，并在损伤后24小时评估外侧和内侧半月板细胞活性。分别于伤后12小时和24小时测定关节组织中一氧化氮(NO)的释放。前交叉韧带和半月板撕裂是由创伤性闭合关节撞击引起的。与内侧半月板和对照肢体相比，创伤性撞击后外侧半月板的细胞活力显著降低。虽然NO在受压关节中的释放量更大，但这种差异在统计学上并不显著。这是第一项研究急性半月板存活后的原位创伤性负荷事件，导致前交叉韧带断裂。外侧半月板细胞活性的改变可能在创伤性膝关节损伤后关节退变的推进中起一定作用。本文的在线版本(DOI：10.1186/1471-2474-15-297)包含补充材料，可供授权用户使用。
Traumatic impaction is known to cause acute cell death and macroscopic damage to cartilage and menisci in vitro. The purpose of this study was to investigate cell viability and macroscopic damage of the medial and lateral menisci using an in situ model of traumatic loading. Furthermore, the release of nitric oxide from meniscus, synovium, cartilage, and subchondral bone was also documented. The left limbs of five rabbits were subjected to tibiofemoral impaction resulting in anterior cruciate ligament (ACL) rupture and meniscal damage. Meniscal tear morphology was assessed immediately after trauma and cell viability of the lateral and medial menisci was assessed 24 hrs post-injury. Nitric oxide (NO) released from joint tissues to the media was assayed at 12 and 24 hrs post injury. ACL and meniscal tearing resulted from the traumatic closed joint impact. A significant decrease in cell viability was observed in the lateral menisci following traumatic impaction compared to the medial menisci and control limbs. While NO release was greater in the impacted joints, this difference was not statistically significant. This is the first study to investigate acute meniscal viability following an in situ traumatic loading event that results in rupture of the ACL. The change in cell viability of the lateral menisci may play a role in the advancement of joint degeneration following traumatic knee joint injury. The online version of this article (doi:10.1186/1471-2474-15-297) contains supplementary material, which is available to authorized users.
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