Treadmill exercise induces hippocampal astroglial alterations in rats.
Treadmill exercise induces hippocampal astroglial alterations in rats.
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DOI：
10.1155/2013/709732
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发表时间：
2013
影响因子：
3.1
通讯作者：
Gonçalves CA
中科院分区：
文献类型：
--
作者：
Bernardi C;Tramontina AC;Nardin P;Biasibetti R;Costa AP;Vizueti AF;Batassini C;Tortorelli LS;Wartchow KM;Dutra MF;Bobermin L;Sesterheim P;Quincozes-Santos A;de Souza J;Gonçalves CA
体育锻炼对大脑健康和认知表现的影响已有描述。在动物模型中已经描述了体育锻炼诱导的海马体突触重塑，但潜在机制仍知之甚少。星形胶质细胞（参与突触重塑的神经胶质细胞）的变化需要更多的特征描述。我们研究了适度的跑步机运动（每天20分钟）持续4周对大鼠海马脑片星形胶质细胞活动的一些参数的影响，即胶质纤维酸性蛋白（GFAP）、谷氨酸摄取和谷氨酰胺合成酶（GS）活性、谷胱甘肽含量、S100B蛋白含量和分泌，以及该组织中脑源性神经营养因子（BDNF）水平和葡萄糖摄取活性。结果表明，适度的跑步机运动能够导致GFAP含量降低（通过酶联免疫吸附测定和免疫组织化学评估）以及GS活性增加。这些变化可能由皮质酮介导，其在血清中的水平升高。另一种假定的介质BDNF在海马组织中没有改变。此外，跑步机运动导致一氧化氮含量降低。我们的数据表明体育锻炼诱导的星形胶质细胞标志物的特定变化，研究星形胶质细胞对于理解大脑可塑性的重要性，以及强化了体育锻炼作为一种神经保护策略的相关性。
Physical exercise effects on brain health and cognitive performance have been described. Synaptic remodeling in hippocampus induced by physical exercise has been described in animal models, but the underlying mechanisms remain poorly understood. Changes in astrocytes, the glial cells involved in synaptic remodeling, need more characterization. We investigated the effect of moderate treadmill exercise (20 min/day) for 4 weeks on some parameters of astrocytic activity in rat hippocampal slices, namely, glial fibrillary acidic protein (GFAP), glutamate uptake and glutamine synthetase (GS) activities, glutathione content, and S100B protein content and secretion, as well as brain-derived neurotrophic factor (BDNF) levels and glucose uptake activity in this tissue. Results show that moderate treadmill exercise was able to induce a decrease in GFAP content (evaluated by ELISA and immunohistochemistry) and an increase in GS activity. These changes could be mediated by corticosterone, whose levels were elevated in serum. BDNF, another putative mediator, was not altered in hippocampal tissue. Moreover, treadmill exercise caused a decrease in NO content. Our data indicate specific changes in astrocyte markers induced by physical exercise, the importance of studying astrocytes for understanding brain plasticity, as well as reinforce the relevance of physical exercise as a neuroprotective strategy.
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影响因子：
5.3
作者：
Haber, Michael;Zhou, Lei;Murai, Keith K.
通讯作者：
Murai, Keith K.
影响因子：
2.9
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Burghardt, PR;Fulk, LJ;Wilson, MA
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Wilson, MA
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Feoli, Ana Maria;Siqueira, Ionara;Goncalves, Carlos-Alberto
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Goncalves, Carlos-Alberto
影响因子：
2.5
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通讯作者：
Meeusen, Romain
影响因子：
56.9
作者：
Beattie, EC;Stellwagen, D;Malenka, RC
通讯作者：
Malenka, RC