Distinct epigenomic landscapes of pluripotent and lineage-committed human cells.
Distinct epigenomic landscapes of pluripotent and lineage-committed human cells.
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DOI：
10.1016/j.stem.2010.03.018
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发表时间：
2010-05-07
期刊：
影响因子：
23.9
通讯作者：
Ren B
中科院分区：
文献类型：
--
作者：
Hawkins RD;Hon GC;Lee LK;Ngo Q;Lister R;Pelizzola M;Edsall LE;Kuan S;Luu Y;Klugman S;Antosiewicz-Bourget J;Ye Z;Espinoza C;Agarwahl S;Shen L;Ruotti V;Wang W;Stewart R;Thomson JA;Ecker JR;Ren B
人类胚胎干细胞（hESCs）与谱系承诺细胞具有相同的基因组，但具有自我更新和多能性的显著特性。不同细胞的不同细胞特性归因于其不同的表观基因组，但表观基因组的差异有多大尚不清楚。在这里，我们报告了hESC和谱系承诺细胞的表观基因组景观是完全不同的。通过比较hESCs和原代成纤维细胞的染色质修饰谱和DNA甲基组，我们发现近三分之一的基因组在染色质结构上存在差异。大多数变化是由抑制H3K9me3和H3K27me3标记的戏剧性重新分布引起的，这些标记在成纤维细胞中形成显著扩大的块。大量潜在的调控序列在染色质修饰和DNA甲基化中也表现出高度的动态性。此外，我们观察到DNA甲基化和染色质修饰之间的新的，上下文依赖的关系。我们的研究结果为多能性和细胞命运承诺的表观遗传机制提供了新的见解。
Human embryonic stem cells (hESCs) share an identical genome with lineage-committed cells, yet possess the remarkable properties of self-renewal and pluripotency. The diverse cellular properties in different cells have been attributed to their distinct epigenomes, but how much epigenomes differ remains unclear. Here, we report that epigenomic landscapes in hESC and lineage committed cells are drastically different. By comparing the chromatin modification profiles and DNA methylomes in hESCs and primary fibroblasts, we find that nearly one-third of the genome differs in chromatin structure. Most changes arise from dramatic redistributions of repressive H3K9me3 and H3K27me3 marks, which form blocks that significantly expand in fibroblasts. A large number of potential regulatory sequences also exhibit a high degree of dynamics in chromatin modifications and DNA methylation. Additionally, we observe novel, context-dependent relationships between DNA methylation and chromatin modifications. Our results provide new insights into epigenetic mechanisms underlying properties of pluripotency and cell-fate commitment.
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影响因子：
46.9
作者：
Ball, Madeleine P.;Li, Jin Billy;Gao, Yuan;Lee, Je-Hyuk;LeProust, Emily M.;Park, In-Hyun;Xie, Bin;Daley, George Q.;Church, George M.
通讯作者：
Church, George M.
影响因子：
64.8
作者：
Heintzman, Nathaniel D.;Hon, Gary C.;Hawkins, R. David;Kheradpour, Pouya;Stark, Alexander;Harp, Lindsey F.;Ye, Zhen;Lee, Leonard K.;Stuart, Rhona K.;Ching, Christina W.;Ching, Keith A.;Antosiewicz-Bourget, Jessica E.;Liu, Hui;Zhang, Xinmin;Green, Roland D.;Lobanenkov, Victor V.;Stewart, Ron;Thomson, James A.;Crawford, Gregory E.;Kellis, Manolis;Ren, Bing
通讯作者：
Ren, Bing
影响因子：
64.5
作者：
Kim, Tae Hoon;Abdullaev, Ziedulla K.;Ren, Bing
通讯作者：
Ren, Bing
影响因子：
7
作者：
Cuddapah, Suresh;Jothi, Raja;Zhao, Keji
通讯作者：
Zhao, Keji
影响因子：
64.8
作者：
Jackson, JP;Lindroth, AM;Jacobsen, SE
通讯作者：
Jacobsen, SE