Sequence Analysis of the Fusion Protein Gene of Human Respiratory Syncytial Virus Circulating in China from 2003 to 2014
Sequence Analysis of the Fusion Protein Gene of Human Respiratory Syncytial Virus Circulating in China from 2003 to 2014
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2003-2014年中国流行的人呼吸道合胞病毒融合蛋白基因序列分析
DOI：
10.1038/s41598-018-35894-3
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Wenbo Xu
中科院分区：
文献类型：
--
作者：
Jinhua Song;Huiling Wang;Teresa I. Ng;Aili Cui;Shuangli Zhu;Yanzhi Huang;Liwei Sun;Zifeng Yang;Deshan Yu;Pengbo Yu;Hong Zhang;Yan Zhang;Wenbo Xu
人呼吸道合胞病毒（human respiratory syncytial virus，HRSV）融合蛋白（F）在HRSV感染中起着重要作用，但对F基因的遗传多样性研究较少。本研究对2003 - 2014年间从中国不同地区采集的330个HRSV F序列进行了分析，以了解其遗传特征。并与Genbank中18个国家的1150个HRSV F基因序列进行了比较。在系统发育分析中，中国HRSV F序列分为许多簇，其中包括来自中国以及其他国家的序列。在基于F基因序列生成的系统发育树中，HRSV亚组内来自不同基因型（基于G基因序列确定）的F序列可以在相同的聚类中找到。氨基酸序列分析表明，中国和其他国家的HRSV F序列高度保守。值得注意的是，所有中国样本的F蛋白序列在帕利珠单抗结合位点完全保守，因此预测了这些菌株对该中和抗体的敏感性。综上所述，2003 - 2014年中国HRSV F序列与其他国家相似，高度保守。
The human respiratory syncytial virus (HRSV) fusion (F) protein is important for HRSV infection, but few studies have examined the genetic diversity of the F gene from Chinese samples. In this study, a total of 330 HRSV F sequences collected from different regions of China between 2003 and 2014 were analyzed to understand their genetic characteristics. In addition, these sequences were compared with 1150 HRSV F sequences in Genbank from 18 other countries. In phylogenetic analysis, Chinese HRSV F sequences sorted into a number of clusters containing sequences from China as well as other countries. F sequences from different genotypes (as determined based on the G gene sequences) within a HRSV subgroup could be found in the same clusters in phylogenetic trees generated based on F gene sequences. Amino acid analysis showed that HRSV F sequences from China and other countries were highly conserved. Of interest, F protein sequences from all Chinese samples were completely conserved at the palivizumab binding site, thus predicting the susceptibility of these strains to this neutralizing antibody. In conclusion, HRSV F sequences from China between 2003 and 2014, similar to those from other countries, were highly conserved.
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影响因子：
3.7
作者：
Xu S;Zhang Y;Zhu Z;Liu C;Mao N;Ji Y;Wang H;Jiang X;Li C;Tang W;Feng D;Wang C;Zheng L;Lei Y;Ling H;Zhao C;Ma Y;He J;Wang Y;Li P;Guan R;Zhou S;Zhou J;Wang S;Zhang H;Zheng H;Liu L;Ma H;Guan J;Lu P;Feng Y;Zhang Y;Zhou S;Xiong Y;Ba Z;Chen H;Yang X;Bo F;Ma Y;Liang Y;Lei Y;Gu S;Liu W;Chen M;Featherstone D;Jee Y;Bellini WJ;Rota PA;Xu W
通讯作者：
Xu W
影响因子：
3.7
作者：
Hause AM;Henke DM;Avadhanula V;Shaw CA;Tapia LI;Piedra PA
通讯作者：
Piedra PA
影响因子：
4.6
作者：
Chen X;Xu B;Guo J;Li C;An S;Zhou Y;Chen A;Deng L;Fu Z;Zhu Y;Liu C;Xu L;Wang W;Shen K;Xie Z
通讯作者：
Xie Z
DOI：
10.1126/science.1234914
发表时间：
2013-05-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
McLellan JS;Chen M;Leung S;Graepel KW;Du X;Yang Y;Zhou T;Baxa U;Yasuda E;Beaumont T;Kumar A;Modjarrad K;Zheng Z;Zhao M;Xia N;Kwong PD;Graham BS
通讯作者：
Graham BS
影响因子：
3.7
作者：
Crowe, JE;Firestone, CY;Murphy, BR
通讯作者：
Murphy, BR