A novel behavioral paradigm for assessing the concept of nests in mice.
A novel behavioral paradigm for assessing the concept of nests in mice.
复制标题
评估小鼠巢概念的新行为范式
DOI：
10.1016/j.jneumeth.2010.03.025
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发表时间：
2010-06-15
影响因子：
3
通讯作者：
Tsien, Joe Z.
中科院分区：
文献类型：
--
作者：
Kuang, Hui;Mei, Bing;Cui, Zhenzhong;Lin, Longnian;Tsien, Joe Z.
大脑中的抽象概念使人类能够有效、正确地识别和分类看似无穷无尽的物体和日常事件。这种抽象的概括能力传统上被认为是人类和非人类灵长类动物所独有的。然而，新兴的神经生理学记录表明，在小鼠大脑中存在着与巢的抽象概念相关的神经。为了便于对小鼠模型中概念的分子和遗传分析，我们基于小鼠的自然行为开发了一个巢概化测试。我们发现，诱导性和前脑特异性NMDA受体敲除在该测试中导致明显的损伤。有趣的是，随着时间的推移，即使面对物体识别记忆的持续缺陷，这种泛化缺陷也可以通过重复的经历逐渐弥补。相比之下，具有微妙表型的前脑特异性早老素-1敲除小鼠在进行该测试时正常。因此，我们的研究不仅建立了一种定量评估小鼠巢概念的方法，而且在结合强大的小鼠遗传学来解剖大脑概念形成的分子基础方面显示了它的巨大潜力。
Abstract concepts in the brain enable humans to efficiently and correctly recognize and categorize a seemingly infinite amount of objects and daily events. Such abstract generalization abilities are traditionally considered to be unique to humans and perhaps non-human primates. However, emerging neurophysiological recordings indicate the existence of neural correlates for the abstract concept of nests in the mouse brain. To facilitate the molecular and genetic analyses of concepts in the mouse model, we have developed a nest generalization test based on mice’s natural behavior. We show that inducible and forebrain-specific NMDA receptor knockout results in pronounced impairment in this test. Interestingly, this generalization deficit could be gradually compensated for over time by repeated experiences even in face of the continued deficit in object recognition memory. On the contrast, the forebrain-specific presenilin-1 knockout mice, which have subtle phenotypes, were normal in performing this test. Therefore, our study not only establishes a quantitative method for assessing the nest concept in mice, but also demonstrates its great potential in combining powerful mouse genetics for dissecting the molecular basis of concept formation in the brain.
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影响因子：
64.8
作者：
Tang, YP;Shimizu, E;Tsien, JZ
通讯作者：
Tsien, JZ
DOI：
10.1073/pnas.0701106104
发表时间：
2007-04-03
影响因子：
11.1
作者：
Lin, Longnian;Chen, Guifen;Tsien, Joe Z.
通讯作者：
Tsien, Joe Z.
影响因子：
3.5
作者：
Luo, J;Niki, K
通讯作者：
Niki, K
影响因子：
64.8
作者：
Quiroga, RQ;Reddy, L;Fried, I
通讯作者：
Fried, I
影响因子：
3
作者：
Tsien, Joe Z.
通讯作者：
Tsien, Joe Z.