Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice.
Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice.
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DOI：
10.1016/j.vaccine.2014.04.016
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发表时间：
2014-05-30
期刊：
影响因子：
5.5
通讯作者：
Frieman, Matthew B.
中科院分区：
文献类型：
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作者：
Coleman, Christopher M.;Liu, Ye V.;Mu, Haiyan;Taylor, Justin K.;Massare, Michael;Flyer, David C.;Glenn, Gregory M.;Smith, Gale E.;Frieman, Matthew B.
关键词：
中东呼吸综合征冠状病毒的出现表明需要针对冠状病毒的新型疫苗策略。含MERS-CoV和SARS-CoV全刺突蛋白的新型纳米颗粒疫苗的制备。免疫小鼠产生高滴度的中和抗体。疫苗接种与一种新的佐剂，基质M1结合，提高中和抗体滴度。制定针对新出现病原体的疫苗接种战略尤其具有挑战性，因为它们的出现具有突发性，而传统疫苗开发需要漫长的过程。因此，有必要开发一种能够在短时间内对新出现的病原体作出反应的快速疫苗开发方法。2003年出现的严重急性呼吸综合征冠状病毒（SARS-CoV）和2012年底出现的中东呼吸综合征冠状病毒（MERS-CoV）表明了冠状病毒作为新发病原体的重要性。冠状病毒的刺突糖蛋白位于病毒粒子表面，负责病毒进入。刺突糖蛋白是冠状病毒的主要免疫优势抗原，已被证明是疫苗设计的一个极好的靶标，旨在阻止冠状病毒进入并促进抗体靶向感染细胞。针对冠状病毒的疫苗接种策略涉及减毒活病毒、重组病毒、表达冠状病毒蛋白的非复制性病毒样颗粒或表达冠状病毒基因的DNA质粒。自SARS-CoV出现以来的十年里，这些策略都没有进展到获得批准的人类冠状病毒疫苗。在这里，我们描述了一种产生MERS-CoV和SARS-CoV全长刺状纳米颗粒的新方法，该方法与佐剂结合能够在小鼠中产生高效价抗体。
Emergence of MERS-CoV demonstrates the need for novel vaccine strategies against coronaviruses. Production of novel nanoparticle vaccine containing full spike protein of MERS-CoV and SARS-CoV. Higher titer neutralizing antibody produced in vaccinated mice. Vaccination in combination with a new adjuvant, Matrix M1, boosts neutralizing antibody titer. Development of vaccination strategies for emerging pathogens are particularly challenging because of the sudden nature of their emergence and the long process needed for traditional vaccine development. Therefore, there is a need for development of a rapid method of vaccine development that can respond to emerging pathogens in a short time frame. The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003 and Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in late 2012 demonstrate the importance of coronaviruses as emerging pathogens. The spike glycoproteins of coronaviruses reside on the surface of the virion and are responsible for virus entry. The spike glycoprotein is the major immunodominant antigen of coronaviruses and has proven to be an excellent target for vaccine designs that seek to block coronavirus entry and promote antibody targeting of infected cells. Vaccination strategies for coronaviruses have involved live attenuated virus, recombinant viruses, non-replicative virus-like particles expressing coronavirus proteins or DNA plasmids expressing coronavirus genes. None of these strategies has progressed to an approved human coronavirus vaccine in the ten years since SARS-CoV emerged. Here we describe a novel method for generating MERS-CoV and SARS-CoV full-length spike nanoparticles, which in combination with adjuvants are able to produce high titer antibodies in mice.
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DOI：
10.1016/s0140-6736(03)13967-0
发表时间：
2003-07-26
期刊：
Lancet (London, England)
影响因子：
--
作者：
Kuiken T;Fouchier RA;Schutten M;Rimmelzwaan GF;van Amerongen G;van Riel D;Laman JD;de Jong T;van Doornum G;Lim W;Ling AE;Chan PK;Tam JS;Zambon MC;Gopal R;Drosten C;van der Werf S;Escriou N;Manuguerra JC;Stöhr K;Peiris JS;Osterhaus AD
通讯作者：
Osterhaus AD
影响因子：
5.5
作者：
Lokugamage KG;Yoshikawa-Iwata N;Ito N;Watts DM;Wyde PR;Wang N;Newman P;Kent Tseng CT;Peters CJ;Makino S
通讯作者：
Makino S
影响因子：
--
作者：
Masters PS
通讯作者：
Masters PS
SARS-COV峰值蛋白的受体结合结构域可诱导高度有效的中和抗体：对开发亚基疫苗的影响。
DOI：
10.1016/j.bbrc.2004.09.106
发表时间：
2004-11-12
影响因子：
3.1
作者：
He Y;Zhou Y;Liu S;Kou Z;Li W;Farzan M;Jiang S
通讯作者：
Jiang S
DOI：
10.1038/nrmicro2090
发表时间：
2009-03
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
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