Age-related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats
Age-related changes in the localization of glycosaminoglycans in condylar cartilage of the mandible in rats
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大鼠下颌骨髁突软骨中糖胺聚糖定位的年龄相关变化
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
H. Mitani
中科院分区：
文献类型：
--
作者：
I. Takahashi;I. Mizoguchi;Y. Sasano;S. Saitoh;M. Ishida;M. Kagayama;H. Mitani
关于下颌髁突软骨的形态学和生物分子特征的信息很少。本研究的目的是确定下颌髁中糖胺聚糖（GAGs）形态和免疫定位的年龄相关变化。采用间接免疫荧光技术检测4、8、16、32和64周龄Wistar雄性大鼠的下颌髁软骨，分别用3种糖胺聚糖、2-B-6、3-B-3和5-D-4单克隆抗体检测硫酸软骨素（Ch-4S）、硫酸软骨素（Ch-6S）和硫酸角蛋白（KS）的定位。形态上，生长时期的髁突软骨为生长软骨，16周龄时从髁突中心区开始分化为关节软骨，32周龄时发育为成熟关节软骨。不同年龄髁前区、中央区、后上区和后区GAGs的形态特征和分布存在区域性差异。这三种糖胺聚糖的免疫组织化学定位显示出年龄相关的、形态依赖的变化，从生长软骨到关节软骨样软骨。所有抗体在区域间基质中的免疫反应均随年龄的增长而逐渐降低，而下颌骨髁突软骨的细胞周基质和区域基质则保持较高的免疫反应性。综上所述，大鼠下颌髁中糖胺聚糖Ch-4S、Ch-6S和KS的定位存在年龄相关和区域差异，这些变化被认为与功能和发育需求有关。
There is little information available regarding the morphological and biomolecular characteristics of mandibular condylar cartilage. The purpose of this study was to determine the age-related changes in the morphology and immunolocalization of glycosaminoglycans (GAGs) in mandibular condyles. The mandibular condylar cartilages from 4-, 8-, 16-, 32-, and 64-week-old Wistar male rats were examined to verify the localization of chondroitin-4-sulfate (Ch-4S), chondroitin-6-sulfate (Ch-6S) and keratan sulfate (KS) using an indirect immunofluorescent technique with three monoclonal antibodies for glycosaminoglycans, 2-B-6, 3-B-3 and 5-D-4, respectively. Morphologically, the condylar cartilage was a growth cartilage during growing periods, began to differentiate into articular cartilage from the central area of 16-week-old condyles, and became mature articular cartilage at 32 weeks of age. A regional difference was found in the morphological features and distribution of GAGs between the anterior, central, postero-superior and posterior areas of the condyles at each age. The immunohistochemical localizations of these three glycosaminoglycans showed age-related, morphology-dependent changes, from growth cartilage to articular cartilage-like cartilage. Immunoreactions for all of the antibodies decreased progressively with age in the interterritorial matrix, while the pericellular and territorial matrix in the condylar cartilage of the mandible maintained relatively higher immunoreactivity. In conclusion, age-related and regional differences in the localization of glycosaminoglycans Ch-4S, Ch-6S, and KS were found in the mandibular condyles in rats, and these changes are believed to be related to functional and developmental requirements.
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人类生长板中糖胺聚糖的免疫定位分析。
DOI：
10.1177/40.2.1372629
发表时间：
1992
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
Byers,S;Caterson,B;Hopwood,JJ;Foster,BK
通讯作者：
Foster,BK
影响因子：
--
作者：
LUDER, HU;LEBLOND, CP;VONDERMARK, K
通讯作者：
VONDERMARK, K
DOI：
10.1007/s40610-015-0019-x
发表时间：
2015
期刊：
Current molecular biology reports
影响因子：
--
作者：
Robinson,Jennifer;O'Brien,Alina;Chen,Jing;Wadhwa,Sunil
通讯作者：
Wadhwa,Sunil
鉴定特异性识别角膜和骨骼硫酸角质素的单克隆抗体。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Caterson,B;Christner,JE;Baker,JR
通讯作者：
Baker,JR
硫酸软骨素/硫酸皮肤素蛋白多糖的表位特异性变化作为发育法氏囊的淋巴细胞和粒细胞生成室中的标记。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sorrell,JM;Lintala,AM;Mahmoodian,F;Caterson,B
通讯作者：
Caterson,B