Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice.
Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice.
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水稻HD-Zip转录因子基因Oshox12和Oshox14的功能分析
DOI：
10.1371/journal.pone.0199248
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ouwerkerk PBF
中科院分区：
文献类型：
--
作者：
Shao J;Haider I;Xiong L;Zhu X;Hussain RMF;Övernäs E;Meijer AH;Zhang G;Wang M;Bouwmeester HJ;Ouwerkerk PBF
同源域-亮氨酸拉链（HD-Zip）转录因子家族在植物发育和形态发生以及对生物和非生物胁迫的响应中起着重要作用。在大麦中，Vrs1 （HvHox1）的隐性突变使二棱大麦变成六棱大麦，这大大提高了产量。Vrs1基因编码一个HD-Zip亚家族I转录因子。系统发育分析表明，水稻HD-Zip I基因Oshox12和Oshox14是Vrs1最接近的同源基因。本研究表明，Oshox12和Oshox14在发育中的穗中普遍表达，且表达水平较高。酵母和水稻原生质体的反式激活实验表明，Oshox12和Oshox14可以结合特定的DNA序列AH1 (CAAT(a /T)ATTG)，激活报告基因的表达。Oshox12和Oshox14在水稻中过表达导致穗长减少和矮化表型。此外，Oshox14过表达系显示出穗外露不足。我们的研究结果表明，Oshox12和Oshox14可能参与了穗发育的调控。本研究为进一步了解HD-Zip转录因子在水稻中的功能提供了重要的进展。
The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.
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