Reciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration.
Reciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration.
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DOI：
10.1016/j.bioactmat.2022.08.021
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发表时间：
2023-03
影响因子：
18.9
通讯作者：
Fu, Xiaobing
中科院分区：
文献类型：
--
作者：
Yuan, Xingyu;Duan, Xianlan;Enhejirigala;Li, Zhao;Yao, Bin;Song, Wei;Wang, Yuzhen;Kong, Yi;Zhu, Shijun;Zhang, Fanliang;Liang, Liting;Zhang, Mengde;Zhang, Chao;Kong, Deling;Zhu, Meifeng;Huang, Sha;Fu, Xiaobing
关键词：
已知脉管系统的并入在组织或器官功能性再生中是有效的。然而，一个模糊的认识之间的相互作用的表皮附属物和他们的血管龛是一个最重要的障碍，以获得汗腺（SG）特定的脉管系统单位。在这里，我们绘制了它们精确的解剖学连接，并报告SG细胞（SGCs）和周围血管生态位之间的相互作用是腺体发育和稳态维持的关键。为了在体外复制这种相互作用，我们使用三维（3D）生物打印从分化的脂肪源性间充质干细胞（ADSC）中生成可重复的SGC球体。以真皮微血管内皮细胞（DMECs）为材料，制备聚己内酯（PCL）牺牲模板，构建血管龛。这种相互作用的模型促进了生理相关的血管化腺体形态发生在体外和体内。我们确定了一个互惠的调节机制，通过接触非依赖性细胞通信和直接的细胞间相互作用的SG和血管系统之间的SG再生。我们设想在不久的将来成功地使用我们的方法进行血管化器官再生。3D结构和PD ECM协同指导ADSC分化为功能性SGCs。SGCs和EC之间的相互作用是腺体稳态和修复的基石。整合SG特异性血管生态位是促进SG再生的有效策略。
The incorporation of vasculature is known to be effective in tissue or organ functional regeneration. However, a vague understanding of the interaction between epidermal appendages and their vascular niches is a foremost obstacle to obtaining sweat gland (SG)-specific vasculature units. Here, we map their precise anatomical connections and report that the interplay between SG cells (SGCs) and the surrounding vascular niche is key for glandular development and homeostasis maintenance. To replicate this interplay in vitro, we used three-dimensional (3D) bioprinting to generate reproducible SGC spheroids from differentiated adipose-derived mesenchymal stem cells (ADSCs). With dermal microvascular endothelial cells (DMECs), sacrificial templates made from poly (ε-caprolactone) (PCL) were fabricated to pattern the vascular niche. This interplay model promoted physiologically relevant vascularized glandular morphogenesis in vitro and in vivo. We identified a reciprocal regulatory mechanism for promoting SGs regeneration via contact-independent cell communication and direct cell-cell interactions between SGs and the vasculature. We envision the successful use of our approach for vascularized organ regeneration in the near future. 3D structure and PD ECM synergistically direct ADSCs differentiation into functional SGCs. The interaction between SGCs and ECs is a cornerstone for glandular homeostasis and repair. Integrating the SG-specific vascular niche is an effective strategy to promote SG regeneration.
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影响因子：
44.1
作者：
Jing D;Zhang S;Luo W;Gao X;Men Y;Ma C;Liu X;Yi Y;Bugde A;Zhou BO;Zhao Z;Yuan Q;Feng JQ;Gao L;Ge WP;Zhao H
通讯作者：
Zhao H
影响因子：
3.7
作者：
Kurata R;Futaki S;Nakano I;Fujita F;Tanemura A;Murota H;Katayama I;Okada F;Sekiguchi K
通讯作者：
Sekiguchi K
影响因子：
4.6
作者：
Kim, Won-Serk;Park, Byung-Soon;Sung, Jong-Hyuk
通讯作者：
Sung, Jong-Hyuk
影响因子：
14.8
作者：
Efremova, Mirjana;Vento-Tormo, Miquel;Vento-Tormo, Roser
通讯作者：
Vento-Tormo, Roser
影响因子：
25
作者：
Cai, Ruiyao;Pan, Chenchen;Ertuerk, Ali
通讯作者：
Ertuerk, Ali