Genomic Landscape of Methylation Islands in Hymenopteran Insects.
Genomic Landscape of Methylation Islands in Hymenopteran Insects.
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DOI：
10.1093/gbe/evy203
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发表时间：
2018-10-01
影响因子：
3.3
通讯作者：
Yi SV
中科院分区：
文献类型：
--
作者：
Jeong H;Wu X;Smith B;Yi SV
最近的昆虫基因组全基因组DNA甲基化分析强调了一个有趣的对比相比，在哺乳动物。在哺乳动物中，除了称为CpG岛的低甲基化位点簇之外，大多数CpG是高度甲基化的。相比之下，昆虫中的DNA甲基化定位于少量CpG位点。在这里，我们将甲基化CpG簇称为“甲基化岛（MIs）”，并利用高质量的亚硫酸氢盐测序数据研究了7种昆虫的特征。甲基化岛主要位于基因体内。它们在外显子-内含子边界中的代表性显着过高，表明它们在剪接中的潜在作用。甲基化的CpG在MI内表现出更强的进化保守性相比，MI外。此外，与不携带MI的基因相比，携带MI的基因表现出更高和更稳定的基因表达水平。MI对进化保守性和基因表达的影响是独立的，并且比单独的DNA甲基化的影响更强。这些结果表明，MI可能是有用的，以获得更多的见解，了解在无脊椎动物基因组中的基因表达和进化保守的DNA甲基化的作用。
Recent genome-wide DNA methylation analyses of insect genomes accentuate an intriguing contrast compared with those in mammals. In mammals, most CpGs are heavily methylated, with the exceptions of clusters of hypomethylated sites referred to as CpG islands. In contrast, DNA methylation in insects is localized to a small number of CpG sites. Here, we refer to clusters of methylated CpGs as “methylation islands (MIs),” and investigate their characteristics in seven hymenopteran insects with high-quality bisulfite sequencing data. Methylation islands were primarily located within gene bodies. They were significantly overrepresented in exon–intron boundaries, indicating their potential roles in splicing. Methylated CpGs within MIs exhibited stronger evolutionary conservation compared with those outside of MIs. Additionally, genes harboring MIs exhibited higher and more stable levels of gene expression compared with those that do not harbor MIs. The effects of MIs on evolutionary conservation and gene expression are independent and stronger than the effect of DNA methylation alone. These results indicate that MIs may be useful to gain additional insights into understanding the role of DNA methylation in gene expression and evolutionary conservation in invertebrate genomes.
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影响因子：
3.9
作者：
Huh I;Zeng J;Park T;Yi SV
通讯作者：
Yi SV
影响因子：
2.6
作者：
Hunt, Brendan G.;Glastad, Karl M.;Goodisman, Michael A. D.
通讯作者：
Goodisman, Michael A. D.
DOI：
10.1534/g3.113.008953
发表时间：
2014-03-20
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Beeler SM;Wong GT;Zheng JM;Bush EC;Remnant EJ;Oldroyd BP;Drewell RA
通讯作者：
Drewell RA
影响因子：
4.4
作者：
Huh I;Yang X;Park T;Yi SV
通讯作者：
Yi SV
影响因子：
5.8
作者：
Capra, John A.;Singh, Mona
通讯作者：
Singh, Mona