Therapeutic hypothermia reduces cortical inflammation associated with utah array implants.
Therapeutic hypothermia reduces cortical inflammation associated with utah array implants.
复制标题
治疗性体温过低可减少与犹他州阵列植入物相关的皮质炎症。
DOI：
10.1088/1741-2552/ab85d2
复制
发表时间：
2020-04-29
影响因子：
4
通讯作者：
Rajguru SM
中科院分区：
文献类型：
--
作者：
Dugan EA;Bennett C;Tamames I;Dietrich WD;King CS;Prasad A;Rajguru SM
神经义肢通过脑机接口（BCI）设备恢复功能具有巨大的前景。然而，考虑到长时间维持神经元信号的挑战以及多种生物机制对电极性能的负面影响，植入式微电极的临床应用仍然有限。急性和慢性炎症、氧化应激和血脑屏障（BBB）破坏导致电极性能不一致。我们假设在微电极插入部位应用治疗性低温（TH）将积极调节炎症和凋亡途径，促进神经保护并长期改善表现。设计了一个定制的装置和热电系统，在微电极阵列插入时和手术后立即将受控的TH局部递送到皮质植入部位。TH模式来源于体内皮层温度测量和皮层温度分布曲线的有限元建模。雄性Sprague-Dawley大鼠植入无功能犹他微电极阵列（UMEA），该微电极阵列由1.5mm长的聚苯乙烯涂层硅柄4×4网格组成。其中一组在UMEA植入后将TH涂抹在植入部位2小时，另一组在常温条件下不进行治疗。在植入后48小时、72小时、7天和14天，比较常温组和低温组炎症和凋亡相关基因mRNA表达水平。定制系统将控制TH输送到皮质种植体部位，数值模型证实温度下降仅限于局部。此外，在UMEA插入后一次性应用TH，通过降低炎症调节细胞因子和趋化因子的表达，显著降低了急性炎症反应。这项工作提供了证据，急性应用低温是有效的显著减少急性炎症后皮质电极植入。
Neuroprosthetics hold tremendous promise to restore function through brain-computer interfaced (BCI) devices. However, clinical applications of implantable microelectrodes remain limited given the challenges of maintaining neuronal signals for extended periods of time and with multiple biological mechanisms negatively affecting electrode performance. Acute and chronic inflammation, oxidative stress, and blood brain barrier (BBB) disruption contribute to inconsistent electrode performance. We hypothesized that therapeutic hypothermia (TH) applied at the microelectrode insertion site will positively modulate both inflammatory and apoptotic pathways, promoting neuroprotection and improved performance in the long-term. A custom device and thermoelectric system were designed to deliver controlled TH locally to the cortical implant site at the time of microelectrode array insertion and immediately following surgery. The TH paradigm was derived from in vivo cortical temperature measurements and finite element modeling of temperature distribution profiles in the cortex. Male Sprague-Dawley rats were implanted with non-functional Utah microelectrodes arrays (UMEA) consisting of 4×4 grid of 1.5mm long parylene-coated silicon shanks. In one group, TH was applied to the implant site for two hours following the UMEA implantation, while the other group was implanted under normothermic conditions without treatment. At 48 hours, 72 hours, 7 days and 14 days post-implantation, mRNA expression levels for genes associated with inflammation and apoptosis were compared between normothermic and hypothermia-treated groups. The custom system delivered controlled TH to the cortical implant site and the numerical models confirmed that the temperature decrease was confined locally. Furthermore, a one-time application of TH post UMEA insertion significantly reduced the acute inflammatory response with a reduction in the expression of inflammatory regulating cytokines and chemokines. This work provides evidence that acutely applied hypothermia is effective in significantly reducing acute inflammation post intracortical electrode implantation.
登录
查看更多内容
影响因子：
2.7
作者：
Alva, N;Carbonell, T;Palomeque, J
通讯作者：
Palomeque, J
影响因子：
3.8
作者：
Diao, CG;Zhu, L;Wang, H
通讯作者：
Wang, H
DOI：
10.1109/tnsre.2017.2677443
发表时间：
2017-10
期刊：
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society
影响因子：
--
作者：
Brandman DM;Cash SS;Hochberg LR
通讯作者：
Hochberg LR
影响因子：
14
作者：
Bennett C;Samikkannu M;Mohammed F;Dietrich WD;Rajguru SM;Prasad A
通讯作者：
Prasad A
影响因子：
6.3
作者：
CLIFTON, GL;JIANG, JY;HAYES, RL
通讯作者：
HAYES, RL