Modulation of airway epithelial antiviral immunity by fungal exposure.
Modulation of airway epithelial antiviral immunity by fungal exposure.
复制标题
通过真菌暴露调节气道上皮抗病毒免疫。
DOI：
10.1165/rcmb.2013-0357oc
复制
发表时间：
2014
影响因子：
6.4
通讯作者：
Chen,Yin
中科院分区：
文献类型：
--
作者：
Zhu,Lingxiang;Lee,Boram;Zhao,Fangkun;Zhou,Xu;Chin,Vanessa;Ling,SerenaC;Chen,Yin
哮喘气道中经常发现多种病原体，如细菌、真菌和病毒，它们与哮喘的发病和加重有关。在这些病原体中，普遍存在的真菌交替孢霉（Alternaria alternata,Alt）和人类鼻病毒已被广泛研究。然而，它们之间的相互作用尚未被研究。在本研究中，我们使用活病毒和合成病毒模拟物双链RNA （dsRNA）测试了Alt暴露对病毒诱导的气道上皮免疫的影响。Alt治疗可显著提高病毒感染或dsRNA治疗诱导的促炎细胞因子（如IL-6和IL-8）的产生。与这种协同作用相反，Alt显著抑制I型和III型IFN的产生，这种损害导致病毒复制升高。机制研究表明，NF-κB和丝裂原活化蛋白激酶途径在抑制IFN产生的TBK1-IRF3途径的协同作用和衰减中发挥了积极作用。这些相反的效果是由不同的真菌成分引起的。蛋白酶依赖性和非依赖性机制似乎参与其中。因此，Alt暴露改变了气道上皮对病毒感染的免疫，转向更炎症但更少抗病毒反应。
Multiple pathogens, such as bacteria, fungi, and viruses, have been frequently found in asthmatic airways and are associated with the pathogenesis and exacerbation of asthma. Among these pathogens,Alternaria alternata(Alt), a universally present fungus, and human rhinovirus have been extensively studied. However, their interactions have not been investigated. In the present study, we tested the effect of Alt exposure on virus-induced airway epithelial immunity using live virus and a synthetic viral mimicker, double-stranded RNA (dsRNA). Alt treatment was found to significantly enhance the production of proinflammatory cytokines (e.g., IL-6 and IL-8) induced by virus infection or dsRNA treatment. In contrast to this synergistic effect, Alt significantly repressed type I and type III IFN production, and this impairment led to elevated viral replication. Mechanistic studies suggested the positive role of NF-κB and mitogen-activated protein kinase pathways in the synergism and the attenuation of the TBK1-IRF3 pathway in the inhibition of IFN production. These opposite effects are caused by separate fungal components. Protease-dependent and -independent mechanisms appear to be involved. Thus, Alt exposure alters the airway epithelial immunity to viral infection by shifting toward more inflammatory but less antiviral responses.
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DOI：
10.1152/ajplung.2001.281.3.l556
发表时间：
2001-09
期刊：
American journal of physiology. Lung cellular and molecular physiology
影响因子：
--
作者：
K. Aoshiba;Kimihiko Yasuda;S. Yasui;J. Tamaoki;A. Nagai
通讯作者：
K. Aoshiba;Kimihiko Yasuda;S. Yasui;J. Tamaoki;A. Nagai
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DOI：
10.1183/09031936.97.10102398
发表时间：
1997
期刊：
The European respiratory journal
影响因子：
--
作者：
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通讯作者：
Chang,MM
DOI：
10.1164/ajrccm.155.4.9105079
发表时间：
1997-04-01
影响因子：
24.7
作者：
Halonen, M;Stern, DA;Martinez, FD
通讯作者：
Martinez, FD
影响因子：
4
作者：
Traves SL;Proud D
通讯作者：
Proud D
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DOI：
10.1016/s1081-1206(10)60635-7
发表时间：
2007
期刊：
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
影响因子：
--
作者：
M. Anthracopoulos;Eva Mantzouranis;A. Paliatsos;G. Tzavelas;E. Lagona;P. Nicolaidou;K. Priftis
通讯作者：
K. Priftis