Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.).
Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.).
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DOI：
10.1038/s41598-018-29068-4
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发表时间：
2018-07-23
影响因子：
4.6
通讯作者：
Tucker MR
中科院分区：
文献类型：
--
作者：
Aubert MK;Coventry S;Shirley NJ;Betts NS;Würschum T;Burton RA;Tucker MR
The aleurone is a critical component of the cereal seed and is located at the periphery of the starchy endosperm. During germination, the aleurone is responsible for releasing hydrolytic enzymes that degrade cell wall polysaccharides and starch granules, which is a key requirement for barley malt production. Inter- and intra-species differences in aleurone layer number have been identified in the cereals but the significance of this variation during seed development and germination remains unclear. In this study, natural variation in mature aleurone features was examined in a panel of 33 Hordeum vulgare (barley) genotypes. Differences were identified in the number of aleurone cell layers, the transverse thickness of the aleurone and the proportion of aleurone relative to starchy endosperm. In addition, variation was identified in the activity of hydrolytic enzymes that are associated with germination. Notably, activity of the free fraction of β-amylase (BMY), but not the bound fraction, was increased at grain maturity in barley varieties possessing more aleurone. Laser capture microdissection (LCM) and transcriptional profiling confirmed that HvBMY1 is the most abundant BMY gene in developing grain and accumulates in the aleurone during early stages of grain fill. The results reveal a link between molecular pathways influencing early aleurone development and increased levels of free β-amylase enzyme, potentially highlighting the aleurone as a repository of free β-amylase at grain maturity.
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影响因子：
1.2
作者：
Gamel, Tamer H.;Abdel-Aal, El-Sayed M.
通讯作者：
Abdel-Aal, El-Sayed M.
影响因子：
5.6
作者：
Burton RA;Fincher GB
通讯作者：
Fincher GB
影响因子：
5.6
作者：
Betts NS;Wilkinson LG;Khor SF;Shirley NJ;Lok F;Skadhauge B;Burton RA;Fincher GB;Collins HM
通讯作者：
Collins HM
影响因子：
6.9
作者：
Hands P;Kourmpetli S;Sharples D;Harris RG;Drea S
通讯作者：
Drea S
影响因子：
--
作者：
Becraft, Philip W.;Gutierrez-Marcos, Jose
通讯作者：
Gutierrez-Marcos, Jose