New class of gene-termini-associated human RNAs suggests a novel RNA copying mechanism.
New class of gene-termini-associated human RNAs suggests a novel RNA copying mechanism.
复制标题
作者：
使用各种技术对人类细胞的短（< 200 nt）RNA（sRNA）谱分析证明了sRNA的意想不到的复杂性，其中存在数十万种sRNA种类。遗传和体外研究表明，这些RNA不仅仅是较长转录本的降解产物，而且可能确实具有功能。此外，RNA（包括sRNA）的谱分析不仅可以揭示新的转录本，而且可以明确预测细胞中新的生化途径的存在和性质。例如，人类细胞中的短RNA分析表明存在一种未知的加帽机制，作用于切割的RNA，其生化成分后来被鉴定出来。在这里，我们表明，人类细胞含有一种新型的sRNA，具有非基因组编码的5' polyU尾巴。这些RNA存在于基因的末端，特别是在已知mRNA的3'末端，这有力地证明了在可以复制RNA的细胞中存在尚未表征的内源性生物化学途径。我们表明，这一途径可以在多个基因上运作，对转录本编码的翻译机器组件的特定富集。最后，我们表明，基因两侧也有正义，3'多聚腺苷酸化的sRNA，可能是加帽的。
Short (< 200 nt) RNA (sRNA) profiling of human cells using various technologies demonstrates unexpected complexity of sRNAs with 100’s of thousands of sRNA species present. Genetic and in vitro studies argue that these RNAs are not merely degradation products of longer transcripts but could indeed have function. Furthermore, profiling of RNAs, including the sRNAs, can reveal not only novel transcripts, but also make clear predictions about the existence and properties of novel biochemical pathways operating in a cell. For example, short RNA profiling in human cells suggested existence of an unknown capping mechanism operating on cleaved RNA a biochemical component of which was later identified. Here we show that human cells contain a novel type of sRNAs that have non-genomically encoded 5’ polyU tails. Presence of these RNAs at the termini of genes, specifically at the very 3’ ends of known mRNAs strongly argues for the presence of a yet uncharacterized endogenous biochemical pathway in a cell that can copy RNA. We show that this pathway can operate on multiple genes, with specific enrichment towards transcripts encoding components of the translational machinery. Finally we show that genes are also flanked by sense, 3’ polyadenylated sRNAs that are likely to be capped.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1126/science.1162253
发表时间：
2008-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Seila AC;Calabrese JM;Levine SS;Yeo GW;Rahl PB;Flynn RA;Young RA;Sharp PA
通讯作者：
Sharp PA
影响因子：
56.9
作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
通讯作者：
Gingeras, Thomas R.
DOI：
10.1073/pnas.93.6.2476
发表时间：
1996-03-19
影响因子：
11.1
作者：
Volloch, V;Schweitzer, B;Rits, S
通讯作者：
Rits, S
影响因子：
64.8
作者：
Rassoulzadegan, Minoo;Grandjean, Valerie;Cuzin, Francois
通讯作者：
Cuzin, Francois