Voltammetric Application of Polypyrrole-Modified Microelectrode Array for the Characterization of DNA Methylation in Glutathione S-Transferase Pi 1.
Voltammetric Application of Polypyrrole-Modified Microelectrode Array for the Characterization of DNA Methylation in Glutathione S-Transferase Pi 1.
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DOI：
10.1080/00032719.2018.1437623
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发表时间：
2018
影响因子：
2
通讯作者：
Saheb A
中科院分区：
文献类型：
--
作者：
Meronard K;Josowicz M;Saheb A
报道了一种直接、高效的无标记伏安法检测谷胱甘肽S-转移酶Pi 1（GSTP 1）超甲基化的方法。微电极阵列芯片用于试纸配置，允许在仅0.35 mL的溶液体积中检测DNA杂交。在与寡核苷酸进行任何接触之前，直径为30 µm的铂微电极盘（n = 16）已用聚吡咯双层进行修饰。15-mer探针DNA与双层的连接是随机的，但受脂肪族系链基团的存在控制，允许其与探针DNA形成双齿复合物。甲基化GST 1特异性靶DNA的伏安检测程序与靶DNA的亚硫酸氢盐处理相结合。在阵列配置中的改性微电极的界面处的变化用于同时记录所有装置上的循环伏安法。通过比较暴露于靶DNA之前和之后记录的循环伏安图中的变化，对杂交的检测进行统计学评估，以确定是或否事件。甲基化靶标的所有循环伏安图显示出比具有非甲基化靶标暴露的那些更大的百分比变化，并且在甲基化靶标暴露后显示出更大的循环伏安图面积变化。我们观察到平均百分比差异为25.6% ± 4.9，变异为19.1%。这些结果表明，快速传感策略具有灵敏度和良好的特异性。此外，该技术可能支持前列腺癌患者的快速，准确诊断和风险评估。
Direct and efficient label-free voltammetric detection of Glutathione S-Transferase Pi 1 (GSTP1) hypermethylation is reported using a custom developed 16-channel Microelectrode Array chip. The microelectrode array chip is used in a dipstick configuration allowing detection of DNA hybridization in a solution volume of only 0.35 mL. Platinum microelectrode disks (n = 16) 30 µm in diameter have been modified with a polypyrrole bilayer before any contact with the oligonucleotides. The attachment of the 15-mer Probe DNA to the bilayer is random but controlled by the presence of aliphatic tether groups allowing it to form a bidentate complex with the probe DNA. The voltammetric detection procedure of methylated GSTP1-specific target DNA is combined with bisulfite treatment of target DNA. Changes at the interface of the modified microelectrodes in an array configuration are used to record simultaneously cyclic voltammetry on all of the devices. The detection of the hybridization is evaluated statistically for a yes or no event by comparing the changes in recorded cyclic voltammograms before and after exposure to the Target DNA. All cyclic voltammograms of the methylated target show a greater percentage change than those with the non-methylated target exposure and show a greater change in cyclic voltammogram area after methylated target exposure. We observe an average percentage difference of 25.6% ± 4.9 with a variation of 19.1%. These results demonstrate that the fast sensing strategy possesses sensitivity and good specificity. Furthermore, this technology can potentially support rapid, accurate diagnosis and risk assessment of patients with prostate cancer.
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DOI：
10.1073/pnas.93.18.9821
发表时间：
1996-09-03
影响因子：
11.1
作者：
Herman, JG;Graff, JR;Baylin, SB
通讯作者：
Baylin, SB
DOI：
10.3390/s90403122
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
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作者：
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通讯作者：
Lanfranchi G
影响因子：
6.6
作者：
Harden, SV;Guo, Z;Sidransky, D
通讯作者：
Sidransky, D
影响因子：
4
作者：
MARTIENSSEN, RA;RICHARDS, EJ
通讯作者：
RICHARDS, EJ
DOI：
10.1016/s1871-0069(05)01009-8
发表时间：
2005-01-01
期刊：
ELECTROCHEMISTRY OF NUCLEIC ACIDS AND PROTEINS: TOWARDS ELECTROCHEMICAL SENSORS FOR GENOMICS AND PROTEOMICS
影响因子：
--
作者：
Aiyejorun, Temitope;Thompson, Liz;Janata, Jiri
通讯作者：
Janata, Jiri