Genome-wide analysis of the Chinese cabbage IQD gene family and the response of BrIQD5 in drought resistance
Genome-wide analysis of the Chinese cabbage IQD gene family and the response of BrIQD5 in drought resistance
复制标题
大白菜IQD基因家族全基因组分析及BrIQD5的抗旱响应
DOI：
10.1007/s11103-019-00839-5
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发表时间：
2019-02
影响因子：
5.1
通讯作者：
Li Ying
中科院分区：
文献类型：
--
作者：
Yuan Jingping;Liu Tongkun;Yu Zhanghong;Li Yan;Ren Haibo;Hou Xilin;Li Ying
关键信息在大白菜中鉴定并分析了35个IQD基因，BrIQD 5转基因植株增强了植物的抗旱性。然而，IQD基因在大白菜对非生物胁迫响应中的作用尚不清楚。本研究在大白菜（Brassica rapassp.pekinensis）中鉴定了BrIQD 1 ~ BrIQD 35共35个IQD基因。基于系统发育分析，这些基因被聚为3个亚家族（I-III），同一亚家族内的成员具有保守的外显子-内含子分布和基序组成。35个BrIQD基因在10条染色体中的9条上分布不均匀，有4个片段重复事件。Ka/Ks比值表明，重复的BrIQDs主要经历了强烈的纯化选择。实时荧光定量聚合酶链反应结果表明，PEG 6000对35个BrIQDs有明显的诱导作用。为了验证BrIQD 5的功能，BrIQD 5在烟草中异源过表达，而在大白菜中沉默。BrIQD 5过表达的植株比野生型植株表现出更强的耐旱性，而BrIQD 5沉默的植株表现出更低的耐旱性。此外，通过酵母双杂交分析，分离出6个BrIQD 5潜在的相互作用蛋白，包括BrCaMa，BrCaMb和其他四个应激相关蛋白。BrIQD 5的基序IQ 1对于与BrCaMa和BrCaMb的相互作用很重要，基序IQ 1中的异亮氨酸是钙调素与BrIQD 5结合的必需氨基酸。大白菜耐旱新基因的鉴定和克隆将促进大白菜的抗旱育种，并有助于更好地理解IQD参与植物耐旱性的机制。
AbstractKey messageThirty-five IQD genes were identified and analysed in Chinese cabbage andBrIQD5transgenic plants enhanced the drought resistance of plants.AbstractThe IQD (IQ67-domain) family plays an important role in various abiotic stress responses in plant species. However, the roles of IQD genes in the Chinese cabbage response to abiotic stress remain unclear. Here, 35 IQD genes, fromBrIQD1toBrIQD35, were identified in Chinese cabbage (Brassica rapassp.pekinensis). Based on the phylogenetic analysis, these genes were clustered into three subfamilies (I-III), and members within the same subfamilies shared conserved exon–intron distribution and motif composition. The 35 BrIQD genes were unevenly distributed on 9 of the 10 chromosomes with 4 segmental duplication events. Ka/Ks ratios showed that the duplicated BrIQDs had mainly experienced strong purifying selection. Quantitative real-time polymerase chain reaction of 35 BrIQDs under PEG6000 indicated thatBrIQD5was significantly induced by PEG6000. To verifyBrIQD5function,BrIQD5was heterologously overexpressed in tobacco and was silenced in Chinese cabbage. BrIQD5-overexpressed plants showed more tolerance to drought stress than wild-type plants, while BrIQD5-silenced plants in Chinese cabbage showed decreased drought tolerance. Additionally, six BrIQD5 potential interactive proteins were isolated by the yeast two-hybrid assay, including BrCaMa, BrCaMb and four other stress-related proteins. Motif IQ1 of BrIQD5 is important for the interaction with BrCaMa and BrCaMb, and the isoleucine in motif IQ1 is an essential amino acid for calmodulin binding to BrIQD5. The identification and cloning of the new Chinese cabbage drought tolerance genes will promote the drought-resistant breeding of Chinese cabbage and help to better understand the mechanism of IQD involved in the drought tolerance of plants.
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影响因子：
7.4
作者：
H. Zhen
通讯作者：
H. Zhen
影响因子：
12.3
作者：
Day IS;Reddy VS;Shad Ali G;Reddy AS
通讯作者：
Reddy AS
DOI：
10.1007/s13580-018-0070-7
发表时间：
2018-08
期刊：
Horticulture, Environment, and Biotechnology
影响因子：
--
作者：
J. Park;Jae-Gyeong Yu;Gi-Ho Lee;Young-Doo Park
通讯作者：
J. Park;Jae-Gyeong Yu;Gi-Ho Lee;Young-Doo Park
影响因子：
5.1
作者：
Chen, CN;Chu, CC;Ho, THD
通讯作者：
Ho, THD
DOI：
10.1073/pnas.0501102102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Maere, S;De Bodt, S;Van de Peer, Y
通讯作者：
Van de Peer, Y