Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia

Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia
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病毒诱导PhFTRv基因沉默导致矮牵牛叶片黄绿色并降低耐寒性

DOI:
10.32615/bp.2020.151
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发表时间:
2020-12
期刊:
影响因子:
1.5
通讯作者:
Liu Juanxu
Liu Juanxu
中科院分区:
生物学4区
文献类型:
--
作者:
Sang Lina;Peng Li;Qiu Zhujun;Luo Fei;Chen Guoju;Gao Lili;Yu Yixun;Liu Juanxu

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铁氧还蛋白-硫氧还蛋白还原酶(FTR)是一种铁硫蛋白,在叶绿体的铁氧还蛋白/硫氧还蛋白系统中提供从光化学还原的铁氧还蛋白(FD)到硫氧还蛋白(TRX)的电子。FTR是一种具有可变亚单位(FTRv)和催化亚基(FTRc)的杂二聚体。FTRv的作用还不是很清楚。在矮牵牛中,FTRv为单拷贝基因,命名为PhFTRv。本研究分析了PhFTRv在矮牵牛中的时空表达,发现PhFTRv在叶和茎中的转录水平较高。本研究采用烟草骚动病毒的基因沉默技术。病毒诱导的基因沉默介导的PhFTRv沉默导致矮牵牛叶片大黄绿,开花延迟,耐冷性降低。附加关键词:开花、叶绿体、叶绿素、叶片发育、矮牵牛、光合作用、温度胁迫。2020年6月28日提交,2020年9月27日最后修订,2020年10月12日接受。缩写:CI体内二氧化碳浓度;FD铁氧还蛋白;FTR铁氧还蛋白-硫氧还蛋白还原酶;GS气孔导度;NTRC NADPH依赖的硫氧还蛋白还原酶C;净光合速率;TRV烟草骚动病毒;TRX硫氧还蛋白;VIGS病毒诱导的基因沉默。认可:本研究得到了中国国家自然科学基金(31770737、31870692、31661143047)和国家重点研究发展计划(2018YFD100015)的资助。*通讯作者;电子邮件:juanguliu@scau.edu.cn这是一篇开放获取的文章,根据知识共享BY-NC-ND许可证的条款分发
Ferredoxin-thioredoxin reductase (FTR) is an iron-sulfur protein that supplies electrons from photochemically reduced ferredoxin (Fd) to thioredoxin (Trx) in the ferredoxin/thioredoxin system in chloroplasts. The FTR is a heterodimer with a variable subunit (FTRv) and a catalytic subunit (FTRc). The function of FTRv is not well known. In petunia (Petunia hybrida), FTRv is a single-copy gene, which is named PhFTRv. In this study, the spatio-temporal expression of PhFTRv in petunia was analyzed, and PhFTRv transcription was found to be high in leaves and stems. A tobacco rattle virus gene silencing was used in this study. Virus induced gene silencing-mediated PhFTRv silencing resulted in large yellow-green leaves, delayed flowering, and reduced cold tolerance in petunia plants. Additional key words: flowering, chloroplasts, chlorophyll, leaf development, Petunia hybrida, photosynthesis, temperature stress. Submitted 28 June 2020, last revision 27 September 2020, accepted 12 October 2020. Abbreviations: ci internal CO2 concentration; Fd ferredoxin; FTR ferredoxin-thioredoxin reductase; gs stomatal conductance; NTRC NADPH-dependent thioredoxin reductase C; PN net photosynthetic rate; TRV tobacco rattle virus; Trx thioredoxin; VIGS virus induced gene silencing. Acknowledgements: This research was supported by the National Natural Science Foundation of China (31770737, 31870692 and 31661143047) and the National Key Research and Development Plan (2018YFD100015). * Corresponding author; e-mail: juanxuliu@scau.edu.cn This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence
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