Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains.
Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains.
复制标题
DOI：
10.1021/pr4011684
复制
发表时间：
2014-05-02
影响因子：
4.4
通讯作者：
Marcotte, Edward M.
中科院分区：
文献类型：
--
作者：
Kwon, Taejoon;Huse, Holly K.;Vogel, Christine;Whiteley, Marvin;Marcotte, Edward M.
最近的研究表明，从直链淀粉基因表达的蛋白质的浓度往往是保守的跨生物体，并在更大程度上比相应的mRNA的丰度。然而，这些研究并没有区分进化（例如，序列分歧）和环境（例如，生长条件）对稳态蛋白质和mRNA丰度的调节的影响。在这里，我们系统地研究了转录组和蛋白质组的两个密切相关的铜绿假单胞菌菌株，PAO1和PA14，在相同的实验条件下，从而控制环境的影响。对于703个基因观察鸟枪蛋白质组学和微阵列实验，我们发现，蛋白质与mRNA的比例是高度相关的两个菌株中的直链淀粉基因之间的蛋白质和mRNA丰度的程度相当。尽管PAO1和PA14之间的这种高分子相似性，我们发现，几个代谢，毒力和抗生素抗性基因的差异表达的两种菌株之间，主要是在蛋白质，但不是在mRNA水平。我们的数据表明，mRNA水平的设置后发生的蛋白质丰度调节的效果的大小和方向是保守的细菌菌株之间，是很重要的解释mRNA和蛋白质丰度之间的不一致。
Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here, we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we found that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that the magnitude and direction of the effect of protein abundance regulation occurring after the setting of mRNA levels is conserved between bacterial strains and is important for explaining the discordance between mRNA and protein abundances.
登录
查看更多内容
影响因子：
9.9
作者：
通讯作者：
--
基因组分析表明，铜绿假单胞菌的毒力是组合。
影响因子：
12.3
作者：
Lee DG;Urbach JM;Wu G;Liberati NT;Feinbaum RL;Miyata S;Diggins LT;He J;Saucier M;Déziel E;Friedman L;Li L;Grills G;Montgomery K;Kucherlapati R;Rahme LG;Ausubel FM
通讯作者：
Ausubel FM
影响因子：
4.4
作者：
Gonzalez, Nicolas;Heeb, Stephan;Valverde, Claudio;Kay, Elisabeth;Reimmann, Cornelia;Junier, Thomas;Haas, Dieter
通讯作者：
Haas, Dieter
影响因子：
7
作者：
Kim, Sangtae;Mischerikow, Nikolai;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
56.9
作者：
Gueell, Marc;van Noort, Vera;Serrano, Luis
通讯作者：
Serrano, Luis