Real-Time Assessment of Staphylococcus aureus Biofilm Disruption by Phage-Derived Proteins.
Real-Time Assessment of Staphylococcus aureus Biofilm Disruption by Phage-Derived Proteins.
复制标题
噬菌体衍生的蛋白质破坏金黄色葡萄球菌生物膜的实时评估。
DOI：
10.3389/fmicb.2017.01632
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Rodríguez A
中科院分区：
文献类型：
--
作者：
Gutiérrez D;Fernández L;Martínez B;Ruas-Madiedo P;García P;Rodríguez A
目前的一个研究重点是开发新的工具来去除工业环境中的细菌生物膜。噬菌体编码的蛋白质，如内溶素、病毒粒子相关的肽聚糖水解酶和胞外多糖解聚酶，已被证明是有效的抗这些结构。然而，目前用于鉴定噬菌体衍生蛋白抗生物被膜特性的筛选技术存在着严重的缺陷。本工作的目的是利用快速、可重复、准确的实时细胞分析仪技术筛选和比较四种噬菌体衍生化合物、三种裂解蛋白(LysH5、CHAP-SH3b和HydH5-SH3b)和一种胞外多糖解聚酶(Dpo7)对反复污染食品的金黄色葡萄球菌生物被膜的抑制作用。生物膜处理后产生的数据允许计算不同的抗生物膜参数：(1)去除50%生物膜的最小生物膜浓度(范围为3.5±1.1~6.6±0.5μM)，(2)观察到抗生物膜效应所需的最低浓度(所有蛋白质的∼1.5μM)，以及(3)比抗生物膜活性和生物膜去除百分比，表明LysH5是最好的抗生物膜化合物。总体而言，该技术可用于通过标准化参数快速评估和比较噬菌体抗生物膜蛋白的解聚活性。
A current focus of research is the development of new tools for removing bacterial biofilms in industrial settings. Bacteriophage-encoded proteins, such as endolysins, virion-associated peptidoglycan hydrolases, and exopolysaccharide depolymerases, have been shown to be efficient against these structures. However, the current screening techniques for the identification of antibiofilm properties of phage-derived proteins have important shortcomings. The aim of this work was to use the rapid, reproducible and accurate technology “real-time cell analyzer” for screening and comparing the antibiofilm ability of four phage-derived compounds, three lytic proteins (LysH5, CHAP-SH3b, and HydH5-SH3b) and one exopolysaccharide depolymerase (Dpo7) against Staphylococcus aureus biofilms, which have been associated with recurrent contamination of food products. The data generated after biofilm treatment allowed for the calculation of different antibiofilm parameters: (1) the minimum biofilm eradicating concentration that removes 50% of the biofilm (ranging from 3.5 ± 1.1 to 6.6 ± 0.5 μM), (2) the lowest concentration needed to observe an antibiofilm effect (∼1.5 μM for all the proteins), and (3) the specific antibiofilm activity and the percentage of biofilm removal that revealed LysH5 as the best antibiofilm compound. Overall, this technology might be used to quickly assess and compare by standardized parameters the disaggregating activity of phage antibiofilm proteins.
登录
查看更多内容
影响因子：
4.1
作者：
Fischetti, Vincent A.
通讯作者：
Fischetti, Vincent A.
影响因子：
3.5
作者：
Anand, Sanjeev;Singh, Diwakar
通讯作者：
Singh, Diwakar
DOI：
10.1016/j.ijfoodmicro.2010.04.029
发表时间：
2010-07-15
影响因子：
5.4
作者：
Garcia, Pilar;Martinez, Beatriz;Rodriguez, Ana
通讯作者：
Rodriguez, Ana
影响因子：
2.4
作者：
Myszka, Kamila;Czaczyk, Katarzyna
通讯作者：
Czaczyk, Katarzyna
影响因子：
2.7
作者：
Allison, DG
通讯作者：
Allison, DG