Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography.
Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography.
复制标题
用于固定化人工膜色谱的磷脂酰乙醇胺功能化仿生整体料
DOI：
10.1016/j.jpha.2021.09.002
复制
发表时间：
2022-04
影响因子：
8.8
通讯作者：
Jiang, Zhengjin
中科院分区：
文献类型：
--
作者：
Zhu, Peijie;Chen, Weijia;Wang, Qiqin;Wu, Huihui;Ruan, Meng;Wang, Hongwu;Jiang, Zhengjin
本研究通过原位聚合1-十二烷酰基-2-（11-甲基丙烯酰胺基十一烷酰基）-sn-甘油基-3-磷酸乙醇胺和二甲基丙烯酸乙二醇酯来模拟生物膜环境，制备了一种新型的磷脂基整体柱。该新型整体柱具有良好的物理化学性能，包括柱效、稳定性和渗透性。此外，该仿生整体柱对一系列完整的蛋白质和小分子显示出优异的分离能力。特别是，它表现出良好的潜力，作为替代商业固定化人工膜（IAM）柱（IAM.PC.DD2）用于研究药物-膜相互作用。本研究不仅丰富了IAM固定相的种类，而且为预测磷脂酰乙醇胺相关的药物性质提供了一个简单的模型。研制了一种新型的磷脂酰乙醇胺功能化固定化人工膜整体柱。用于制备聚（MDSPE-co-EDMA）整体柱的聚合混合物的组合物进行了优化，用于微型LC应用。该仿生整体柱对完整蛋白质和小分子物质均表现出良好的分离选择性。该仿生整体柱具有很大的潜力，可替代商业化的IAM柱用于药物-膜相互作用的研究。
In this research, a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane environment. Excellent physicochemical properties of this novel monolith that were achieved included column efficiency, stability, and permeability. Moreover, the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small molecules. In particular, it exhibited good potential as an alternative to the commercial immobilized artificial membrane (IAM) column (IAM.PC.DD2) for studying drug-membrane interactions. This study not only enriched the types of IAM stationary phases, but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates. A novel phosphatidylethanolamine (PE) functionalized immobilized artificial membrane (IAM) monolithic column was developed. The composition of the polymerization mixtures used for the preparation of poly(MDSPE-co-EDMA) monolithic columns was optimized for micro-LC applications. The biomimetic monolith exhibited good separation selectivity for both intact proteins and small molecules. The biomimetic monolith exhibited great potential as a replacement of commercial IAM columns for studying drug-membrane interactions.
登录
查看更多内容
影响因子：
4.6
作者：
Barbato, F;di Martino, G;La Rotonda, MI
通讯作者：
La Rotonda, MI
影响因子：
4.3
作者：
De Vrieze, Mike;Verzele, Dieter;Lynen, Frederic
通讯作者：
Lynen, Frederic
用于加压毛细管电色谱的新型高亲水性甲基丙烯酸酯整体柱，具有亲水性和强阳离子交换相互作用的混合模式
影响因子：
4.1
作者：
Lin, Jia;Liu, Shaofeng;Xie, Zenghong
通讯作者：
Xie, Zenghong
影响因子：
6.8
作者：
Tsopelas, Fotios;Stergiopoulos, Chrysanthos;Tsantili-Kakoulidou, Anna
通讯作者：
Tsantili-Kakoulidou, Anna
影响因子：
4.1
作者：
Jiang, Zhengjin;Smith, Norman William;Liu, Zhenghua
通讯作者：
Liu, Zhenghua