An electrochemical sensor for single nucleotide polymorphism detection in serum based on a triple-stem DNA probe.
An electrochemical sensor for single nucleotide polymorphism detection in serum based on a triple-stem DNA probe.
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DOI：
10.1021/ja905068s
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发表时间：
2009-10-28
影响因子：
15
通讯作者：
Soh, H. Tom
中科院分区：
文献类型：
--
作者：
Xiao, Yi;Lou, Xinhui;Uzawa, Takanori;Plakos, Kory J. I.;Plaxco, Kevin W.;Soh, H. Tom
我们在此报告了一种电化学方法，该方法首次在复杂样品（例如血清）中直接提供单步、室温单核苷酸多态性（SNP）检测，无需目标修饰、后清洗或添加外源试剂。该传感器灵敏、稳定且可重复使用，由具有三茎结构的单一、自互补、亚甲蓝标记的 DNA 探针组成。该探针利用了在结合完美匹配的目标时发生的主要构象重排所导致的热函和熵的巨大热力学变化，从而导致法拉第电流的大规模变化。因此，该传感器的辨别能力大大超过了早期的单杆和双杆电化学传感器，并支持单核苷酸取代的快速（分钟）、单步、无试剂、室温检测。为了阐明传感器选择性的理论基础，我们对单杆、双杆和三杆探针进行了比较热力学分析。
We report here an electrochemical approach that offers, for the first time, single-step, room-temperature single nucleotide polymorphism (SNP) detection directly in complex samples (such as blood serum) without the need for target modification, post-washing or the addition of exogenous reagents. This sensor, which is sensitive, stable and reusable, is comprised of a single, self-complementary, methylene blue-labeled DNA probe possessing a triple-stem structure. This probe takes advantage of the large thermodynamic changes in enthalpy and entropy that result from major conformational rearrangements that take place upon binding a perfectly-matched target, resulting in a large-scale change in the faradaic current. As a result, the discrimination capabilities of this sensor greatly exceed those of earlier single- and double-stem electrochemical sensors, and supports rapid (minutes), single-step, reagentless, room-temperature detection of single nucleotide substitutions. To elucidate the theoretical basis of the sensor’s selectivity, we present a comparative thermodynamic analysis among single-, double- and triple-stem probes.
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影响因子：
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作者：
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