More than meets the eye: associations of vaginal bacteria with gram stain morphotypes using molecular phylogenetic analysis.
More than meets the eye: associations of vaginal bacteria with gram stain morphotypes using molecular phylogenetic analysis.
复制标题
DOI：
10.1371/journal.pone.0078633
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fredricks DN
中科院分区：
文献类型：
--
作者：
Srinivasan S;Morgan MT;Liu C;Matsen FA;Hoffman NG;Fiedler TL;Agnew KJ;Marrazzo JM;Fredricks DN
细菌性阴道病（BV）是一种高度流行的疾病，与不良的健康结果。阴道液的革兰氏染色分析是确认BV诊断的标准，其中评估关键细菌形态型的丰度。这些乳酸杆菌，加德纳菌，拟杆菌，和Mobiluncus形态型最初通过培养研究与特定的细菌物种，但没有研究系统地研究了未培养的细菌之间的关联检测到的分子方法和革兰氏染色结果。在这项研究中，16 S-rRNA PCR/焦磷酸测序被用来检查阴道细菌和细菌形态型之间的关联，在220名妇女与BV和没有。使用种特异性定量PCR（qPCR）和荧光原位杂交（FISH）方法记录两种具有弯曲杆状形态的细菌的浓度：Mobiluncus和苛养BV相关细菌-1（BVAB 1）。在具有弯曲革兰氏阴性杆菌证据的样本中，阴道细菌的等级丰度显示BVAB 1占优势（26.1%），而动弯菌属很少见（占序列读段的0.2%）。BVAB 1序列读段与活动弯藻形态型相关（p<0.001）。使用种特异性定量PCR，在弯曲杆的女性中，BVAB 1 DNA的平均浓度比活动弯高2个对数单位（p<0.001）。FISH分析显示，在Nugent评分最高的女性中，BVAB 1细胞的平均数量比Mobiluncus细胞多2个对数单位（p<0.001）。普氏菌属和卟啉单胞菌属。与“拟杆菌形态型”显著相关，而拟杆菌属物种很少见。在革兰氏染色上被指定为动弯杆菌形态型的革兰氏阴性杆菌更可能是BVAB 1。这些发现提供了与阴道革兰氏染色形态相关的细菌的更清晰的图像。
Bacterial vaginosis (BV) is a highly prevalent condition associated with adverse health outcomes. Gram stain analysis of vaginal fluid is the standard for confirming the diagnosis of BV, wherein abundances of key bacterial morphotypes are assessed. These Lactobacillus, Gardnerella, Bacteroides, and Mobiluncus morphotypes were originally linked to particular bacterial species through cultivation studies, but no studies have systematically investigated associations between uncultivated bacteria detected by molecular methods and Gram stain findings. In this study, 16S-rRNA PCR/pyrosequencing was used to examine associations between vaginal bacteria and bacterial morphotypes in 220 women with and without BV. Species-specific quantitative PCR (qPCR) and fluorescence in Situ hybridization (FISH) methods were used to document concentrations of two bacteria with curved rod morphologies: Mobiluncus and the fastidious BV-associated bacterium-1 (BVAB1). Rank abundance of vaginal bacteria in samples with evidence of curved gram-negative rods showed that BVAB1 was dominant (26.1%), while Mobiluncus was rare (0.2% of sequence reads). BVAB1 sequence reads were associated with Mobiluncus morphotypes (p<0.001). Among women with curved rods, mean concentration of BVAB1 DNA was 2 log units greater than Mobiluncus (p<0.001) using species-specific quantitative PCR. FISH analyses revealed that mean number of BVAB1 cells was 2 log units greater than Mobiluncus cells in women with highest Nugent score (p<0.001). Prevotella and Porphyromonas spp. were significantly associated with the “Bacteroides morphotype,” whereas Bacteroides species were rare. Gram-negative rods designated Mobiluncus morphotypes on Gram stain are more likely BVAB1. These findings provide a clearer picture of the bacteria associated with morphotypes on vaginal Gram stain.
登录
查看更多内容
影响因子：
6.4
作者：
ROBERTS, MC;HILLIER, SL;HOLMES, KK
通讯作者：
HOLMES, KK
影响因子：
2.3
作者：
Matu, Martin N.;Orinda, George O.;Bukusi, Elizabeth A.
通讯作者：
Bukusi, Elizabeth A.
影响因子：
3.6
作者：
Ison, CA;Hay, PE
通讯作者：
Hay, PE
影响因子：
158.5
作者：
Hillier, SL;Nugent, RP;Klebanoff, MA
通讯作者：
Klebanoff, MA
影响因子：
3.1
作者：
Koumans, Emilia H.;Sternberg, Maya;Markowitz, Lauri E.
通讯作者：
Markowitz, Lauri E.