Compositional mapping of the mature anterior cruciate ligament-to-bone insertion.
Compositional mapping of the mature anterior cruciate ligament-to-bone insertion.
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DOI：
10.1002/jor.23539
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Lu HH
中科院分区：
文献类型：
--
作者：
Qu D;Subramony SD;Boskey AL;Pleshko N;Doty SB;Lu HH
前交叉韧带（ACL）-骨界面构成复杂的多组织结构，包括连续韧带、非矿化纤维软骨、矿化纤维软骨和骨区域。这种复合结构能够在结构和功能不同的组织之间转移载荷，对于韧带稳态和关节稳定性至关重要。目前，缺乏定量的了解矩阵的组成和组织跨越这个路口，特别是在骨骼成熟的发病。因此，本研究的目的是使用傅里叶变换红外光谱成像（FTIRI）表征成年牛ACL-骨界面，检验胶原蛋白、蛋白多糖和矿物质分布的区域变化以及基质组织在成熟插入时持续存在的假设。据观察，虽然胶原蛋白含量在成人界面上不断增加，但韧带和骨之间的胶原蛋白排列减少。蛋白聚糖主要定位于纤维软骨区域，并且在非矿化区域和矿化区域之间观察到矿物质含量的指数增加。这些观察结果揭示了胶原分布的显著变化和与成熟度的一致性，这些趋势强调了生理负荷在出生后基质重塑中的作用。这项研究的结果为界面组织提供了新的见解，并作为界面再生和一体化软组织再生的基准设计标准。
The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions. This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability. Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity. Therefore, the objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization persist at the mature insertion. It was observed that while collagen content increases continuously across the adult interface, collagen alignment decreases between ligament and bone. Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions. These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling. Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue regeneration.
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影响因子：
2.9
作者：
Gadaleta, SJ;Landis, WJ;Mendelsohn, R
通讯作者：
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DOI：
10.1073/pnas.0712150105
发表时间：
2008-06-10
影响因子：
11.1
作者：
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影响因子：
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