Genomic Variability among Field Isolates and Laboratory-Adapted Strains of Leptospira borgpetersenii Serovar Hardjo.
Genomic Variability among Field Isolates and Laboratory-Adapted Strains of Leptospira borgpetersenii Serovar Hardjo.
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DOI：
10.1155/2018/2137036
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发表时间：
2018
影响因子：
3.4
通讯作者：
Rajeev S
中科院分区：
文献类型：
--
作者：
Llanes A;Restrepo CM;Riesgo-Ferreiro P;Rajeev S
博格彼得氏钩端螺旋体血清型在牛肾上定植，偶尔可能感染人类和其他哺乳动物。菌株属于两个克隆亚型（A型和B型），它们在仓鼠实验模型中的致病性有显著差异。这种差异归因于个体基因的点突变，尽管这些基因尚未被表征。为了更好地了解博格彼得氏乳杆菌血清型Hardjo分离株的遗传变异性，我们对两株实验室适应菌株和三株屠宰场衍生的博格彼得氏乳杆菌血清型Hardjo进行基因组测序和比较。在同一亚型的分离株中观察到相对较低的遗传变异性，在实验室适应的分离株中发现了大多数中等或高度影响的突变。相比之下，两个亚型之间在基因含量和遗传变异方面存在一些差异。假定的类型特异性基因似乎编码与感染关键功能相关的蛋白质。其中一些基因似乎参与转录调控，可能导致每种类型的不同调控模式。这些结果表明，先前认为在钩端螺旋体形成过程中至关重要的调节机制的变化可能发生在博格彼得螺旋体中。此外，本研究中用于变异召唤的生物信息学方法可用于其他非模式原核生物（如钩端螺旋体）的研究。
Leptospira borgpetersenii serovar Hardjo colonizes cattle kidneys and may occasionally infect humans and other mammals. Strains belonging to two clonal subtypes (types A and B) with marked differences in their pathogenicity in the hamster experimental model have been described for this serovar. Such differences have been attributed to point mutations in individual genes, although those genes have not yet been characterized. In order to better understand genetic variability among L. borgpetersenii serovar Hardjo isolates, we sequenced and compared the genomes of two laboratory-adapted strains and three abattoir-derived field isolates of L. borgpetersenii serovar Hardjo. Relatively low genetic variability was observed within isolates of the same subtype, with most of the mutations of moderate or high impact found in the laboratory-adapted isolates. In contrast, several differences regarding gene content and genetic variants were observed between the two subtypes. Putative type-specific genes appear to encode proteins associated with functions that are critical for infection. Some of these genes seem to be involved in transcriptional regulation, possibly leading to a distinct regulatory pattern in each type. These results show that changes in regulatory mechanisms, previously suggested to be critical during Leptospira speciation, may occur in L. borgpetersenii. In addition, the bioinformatics methodology used in this study for variant calling can be useful to other groups working with nonmodel prokaryotic organisms such as Leptospira species.
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影响因子：
3.7
作者：
Llanes A;Restrepo CM;Rajeev S
通讯作者：
Rajeev S
DOI：
10.1093/bioinformatics/btp347
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Assefa S;Keane TM;Otto TD;Newbold C;Berriman M
通讯作者：
Berriman M
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
2.1
作者：
Aravind, L;Ponting, CP
通讯作者：
Ponting, CP