Differential analogue binding by two classes of c-di-GMP riboswitches.
Differential analogue binding by two classes of c-di-GMP riboswitches.
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DOI：
10.1021/ja204650q
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发表时间：
2011-10-05
影响因子：
15
通讯作者：
Strobel, Scott A.
中科院分区：
文献类型：
--
作者：
Shanahan, Carly A.;Gaffney, Barbara L.;Jones, Roger A.;Strobel, Scott A.
细菌适应不断变化的环境的能力对它们的生存至关重要。细菌已经进化出一种机制，可以感知环境线索，并使用第二个信使信号分子环二鸟苷一磷酸(c-di-GMP)将这些信号转化为表型变化。除了几类蛋白质受体外，两类c-di-GMP结合核糖开关(I类和II类)已被确定为该信号通路中第二信使的下游靶标。与c-di-GMP结合的这两类核糖开关的晶体结构已有报道。在这里，我们进一步研究RNA为识别和结合第二信使而进化的机制。使用一系列c-di-GMP类似物，我们探索了两类核糖开关在RNA-配体复合体中的相互作用，以确定c-di-GMP最关键的结合元件。我们发现，这两个效应器结合所需的c-di-GMP的结构特征不同，并且II类核糖开关在配体结合方面的歧视比I类核糖开关小得多。这些数据为预测在c-di-GMP信号通路中优先使用I类基序而不是II类基序提供了解释。
The ability of bacteria to adapt to a changing environment is essential for their survival. One mechanism bacteria have evolved to sense environmental cues and translate these signals into phenotypic changes uses the second messenger signaling molecule, cyclic diguanosine monophosphate (c-di-GMP). In addition to several classes of protein receptors, two classes of c-di-GMP-binding riboswitches (class I and class II) have been identified as downstream targets of the second messenger in this signaling pathway. The crystal structures of both riboswitch classes bound to c-di-GMP were previously reported. Here we further investigate the mechanisms that RNA has evolved for recognition and binding of this second messenger. Using a series of c-di-GMP analogs, we probed the interactions made in the RNA-ligand complex for both classes of riboswitches to identify the most critical elements of c-di-GMP for binding. We found that the structural features of c-di-GMP required for binding differ between these two effectors and that the class II riboswitch is much less discriminatory in ligand binding than the class I riboswitch. These data suggest an explanation for the predicted preferential use of the class I motif over the class II motif in the c-di-GMP signaling pathway.
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细菌外多糖产生所需的环状-DI-GMP受体。
影响因子：
3.6
作者：
Lee, Vincent T;Matewish, Jody M;Kessler, Jennifer L;Hyodo, Mamoru;Hayakawa, Yoshihiro;Lory, Stephen
通讯作者：
Lory, Stephen
影响因子：
--
作者：
Kiburu, Irene;Shurer, Andrew;Sintim, Herman O.
通讯作者：
Sintim, Herman O.
影响因子：
5.8
作者：
Amikam, D;Galperin, MY
通讯作者：
Galperin, MY
影响因子：
5.2
作者：
Gaffney, Barbara L.;Veliath, Elizabeth;Zhao, Jianwei;Jones, Roger A.
通讯作者：
Jones, Roger A.
影响因子：
64.5
作者：
Boehm, Alex;Kaiser, Matthias;Jenal, Urs
通讯作者：
Jenal, Urs