Compartmentation of photosynthesis gene expression in C4 maize depends on time of day.
Compartmentation of photosynthesis gene expression in C4 maize depends on time of day.
复制标题
C4玉米中光合作用基因表达的区室化取决于一天中的时间。
DOI：
10.1093/plphys/kiad447
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发表时间：
2023-11-22
期刊：
影响因子：
7.4
通讯作者：
Hibberd, Julian M.
中科院分区：
文献类型：
--
作者：
Borba, Ana Rita;Reyna-Llorens, Ivan;Dickinson, Patrick J.;Steed, Gareth;Gouveia, Paulo;Gorska, Alicja M.;Gomes, Celia;Kromdijk, Johannes;Webb, Alex A. R.;Saibo, Nelson J. M.;Hibberd, Julian M.
与祖先的C3状态相比，C4光合作用以更高的速率发生，提高了水和氮的利用效率。在C3和C4植物中，光合作用速率随着光照强度的增加而增加，并且在中午左右达到最大值。我们确定，在没有光或温度波动的情况下，玉米（玉米）的光合作用在主观光周期的中间达到峰值。为了研究与这些时间变化相关的分子过程，我们在24小时的时间过程中进行了玉米叶肉和束鞘链的RNA测序。在这些细胞类型的C4周期基因的优先表达是最强的6和10小时之间的黎明后，当光合速率最高。对于束鞘，DNA基序富集和基因共表达分析表明，与一个手指（DOF）和MADS（微小染色体维持因子1/AGAMOUS/DEFICIENS/血清反应因子）-域转录因子家族的DNA结合的成员介导C4基因表达的昼夜波动，而植物中的反式激活试验证实了它们激活来自束鞘表达基因的启动子片段的能力。因此，这项工作确定了转录调节因子和细胞特异性C4基因表达的峰值，与中午玉米叶片中光合作用的最大速率相一致。C4基因表达在叶肉和维管束鞘股之间的空间分配依赖于一天中的时间。
Compared with the ancestral C3 state, C4 photosynthesis occurs at higher rates with improved water and nitrogen use efficiencies. In both C3 and C4 plants, rates of photosynthesis increase with light intensity and are maximal around midday. We determined that in the absence of light or temperature fluctuations, photosynthesis in maize (Zea mays) peaks in the middle of the subjective photoperiod. To investigate the molecular processes associated with these temporal changes, we performed RNA sequencing of maize mesophyll and bundle sheath strands over a 24-h time course. Preferential expression of C4 cycle genes in these cell types was strongest between 6 and 10 h after dawn when rates of photosynthesis were highest. For the bundle sheath, DNA motif enrichment and gene coexpression analyses suggested members of the DNA binding with one finger (DOF) and MADS (MINICHROMOSOME MAINTENANCE FACTOR 1/AGAMOUS/DEFICIENS/Serum Response Factor)-domain transcription factor families mediate diurnal fluctuations in C4 gene expression, while trans-activation assays in planta confirmed their ability to activate promoter fragments from bundle sheath expressed genes. The work thus identifies transcriptional regulators and peaks in cell-specific C4 gene expression coincident with maximum rates of photosynthesis in the maize leaf at midday. The spatial partitioning of C4 gene expression between mesophyll and bundle sheath strands is dependent on time of day.
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影响因子：
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